APOB Antibody (LS-B2440)

貨號 Cat. No.: LS-B2440
規格 Size: 50 Âµg
品牌 Brand: LSBio

Description

Antibody Catalog ID:	LS-B2440
Antibody Validation:	This antibody replaces catalog number LS-C20722. It has been validated for use in the following assays: IHC-P.
Antibody Target:	APOB
Synonyms:	APOB Antibody, apo b-100 Antibody, apo b-48 Antibody, apolipoprotein b-100 Antibody, apolipoprotein b-48 Antibody, FLDB Antibody, LDLCQ4 Antibody
Host:	APOB antibody was produced in Goat
Antigen Species:	APOB antibody was raised against Human
Clonality:	Polyclonal
Isotype:	IgG
Modifications:	Biotin (Biotin)
Antigen Type:	Purified protein
Immunogen:	APOB antibody was raised against apolipoprotein Type B isolated from human plasma by density gradient centrifugation followed by HPLC purification.
Antibody Specificity:	Recognizes human apolipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J apolipoproteins. Typically less than 1% cross-reactivity against other types of apolipoprotein was detected by ELISA against purified standards. Non-specific cross-reaction of anti-apolipoprotein antibodies with other human serum proteins is negligible.
Purification:	Immunoaffinity Chromatography
Presentation:	PBS, pH 7.2. No preservative added. No stabilizing proteins added.
Recommended Storage:	+4°C or -20°C, Avoid repeated freezing and thawing.
Antibody Usage Summary:	Immunohistochemistry: LS-B2440 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2440 was determined to be 1:25-1:50.
Antibody Uses:	Immunohistochemistry - Paraffin (IHC-P) (1:25 - 1:50), Western blot (WB) (1:1000 - 1:5000), Immunoprecipitation (IP), ELISA (1:1000 - 1:5000) (Optimal dilution to be determined by the researcher)
Size:	50 µg