APO LOGIX - Carboxyfluorescein Caspase-9 Detection Kit

·         Non-cytotoxic assay arrests further apoptotic activity via caspase inhibition.

·         Cell permeablity permits direct visualization of cytosolic apoptotic events.

·         Apoptotic cell population does not diminish over time.

·         Add reagent directly to cells. No special buffer or media needed. No preparation of cell lysates required. Simple wash procedure.

·         Works in diverse cell lines: human, rodent, Drosophila.

·         Can be performed in conjunction with Annexin staining, TUNEL, antibody staining, or with other APO LOGIX reagents on the same population of cells.

·         Permits high through-put screening. Protocol can be adapted for ex vivo as well as in situ experiments.

·         Works with fluorescence microscopy, 96-well fluorescence plate readers, or flow cytometry.

·         Yields both quantitative and qualitative results. Gives strong signal with little background noise.

·         Mark activity across the range of caspase proteins. Poly-caspase and caspase-specific assays to target caspases 1, 2, 3, 6, 8, 9, or 10 are available.

·         Applications - Flow Cytometry, Fluorescence Plate Reader, Fluorescent Microscope

Contents:

·         Lyophilized FAM labeled peptide inhibitor

·         10X Wash Buffer

·         10X Fixative

·         Propidium Iodide