APO ACTIVE 3 Phycoerythrin (PE)

Key Benefits:

·         Highly specific for active human and murine caspase 3. Other assays require the utilization of peptide based (DEVD) reagents that tend to cross-react with caspase 7 and other caspases.

·         Applications - Works with a flow cytometer, flourescence microscope, or 96 well plate reader.

·         Yields both quantitative and qualitative results. Gives a strong positive signal.

·         Can be used in conjunction with other antibodies or stains.

·         No need to make cell lysates or run western blots. Cells can be fixed and analyzed later.

·         Works with human, mouse and rat cell lines.

Assay Principle

Innovative Research’s APO-ACTIVE 3 PE Kit utilizes a rabbit affinity purified polyclonal antibody raised against amino acid 163-175 of murine caspase 3 (9). This neo epitope is present on the p18 subunit of cleaved caspase 3 (9). Cells undergoing apoptosis are fixed and permeablized prior to the addition of the antibody. A secondary PE labeled goat anti rabbit antibody is used to visualize the bound rabbit anti caspase 3 polyclonal. PE labeling allows for use with a variety of applications.

Kit contents

·         1 vial of Rabbit anti cleaved caspase 3 affinity purified polyclonal antibody

·         1 vial of PE labeled Goat anti Rabbit affinity purified polyclonal antibody

·         1 vial of 10X fixative.

Size: 25 Tests