APO 3/7 HTS - High Throughput Screen for Caspase-3/7 Detection

Key Benefits:

·         Homogenous assay for active caspase 3/7.

·         Breakthrough in cell lysis buffer and preservation of caspase activity.

·         Results in a No wash one-step assay

·         No need to wash out media from cell samples, just add the reagent directly to your experimental samples.

·         Easy to Use: No need to make cell lysates or run western blots.

·         Works with suspension and adherent cells.

Applications - Standard Fluorescence Plate Reader 

Assay Principle:

Innovative Research’s Apo 3/7 HTS Assay utilizes the quenched (z-DEVD)₂-R110 peptide substrate for caspase 3/7 detection. The absorption and emission properties of the R110 dye are suppressed when attached to the z-DEVD peptide sequence. When R110 is cleaved away, by active caspase3/7, form the quenching DEVD sequence, the free dye excites at 488nm and emits at 515-530 nm.. As a result of a novel and proprietary Lysis Buffer System, the Apo 3/7 HTS Assay is a homogenous platform that can be utilized for high throughput fluorescence plate reader applications. The reagent is directly added to the samples thus eliminating any wash steps.

Kit contents:

·         1 vial Caspase 3/7 Reagent. (z-DEVD)₂ Rhodamine 110 Part#4004

·         1 Bottle of Cell Lysis Buffer

Size: 100 Tests