APC (Q2829) Antibody

stock

Host: Rabbit

Reactivity: Human,Mouse,Rat

Product

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

APC (Q2829) antibody detects endogenous levels of APC protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

he adenomatous polyposis syndromes, familial adenomatous polyposis(FAP) and Gardner’s syndrome (GS), are characterized by numerous adenomatous polyps throughout the entire colon. These polyps invariably progress to colon cancer in addition to other extracolonic manifestations. The cloning of the APC gene revealed a ubiquitously expressed protein, 2843 amino acids in length with a molecular weight of 300 kD,a which is frequently mutated in patients suffering from FAP and GS. APC has recently been found to be associated with structural components of intracellular junctions. β-catenin and γ-catenin (also called plakoglobin) are involved in the regulation of cellular adhesion. APC and E cadherin compete for binding to specific internal regions of both β- and γ-catenin. Interactions between the cytoskeleton and the APC, E cadherin and β/γ catenin complex are mediated by α-catenin. Like APC, APC2 contains SAMP domains, which are required for conductin binding. Both APC and APC2 regulate the formation of active β-catenin-Tcf complexes..