Apaf-1 Antibody
- 貨號 Cat. No.
- 3018-100
- 規格 Size
- 100 µg
Description
pfont size=2strongAntibody Target :/strongApaf-1 /font/p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide surrounding amino acid 1161 of human Apaf-1/p
pbAccession#: /bO14727/p
pbGene ID: /b20141188/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.2 mg/ml/p
pbFormulation: /b100 micro;g (0.2 mg/ml) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.05% BSA, 0.01% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rtbv/rb/mk/hs/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bDuring apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues./p
pbHost: /bRabbit/p
pbAntibody Type: /bPolyclonal/p
pbIsotype: /bRabbit IgG/p
pbAntibody Form: /bAffinity Purified Antibody/p
pbImmunogen Sequence: /bSynthetic peptide surrounding amino acid 1161 of human Apaf-1/p
pbAccession#: /bO14727/p
pbGene ID: /b20141188/p
pbAppearance: /bColorless liquid/p
pbConcentration: /b0.2 mg/ml/p
pbFormulation: /b100 micro;g (0.2 mg/ml) immunoaffinity purified, rabbit anti-Apaf-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.05% BSA, 0.01% thimerosal./p
pbPurification: /bAffinity purified/p
pbSpecies Reactivity: /bhu/ms/rtbv/rb/mk/hs/p
pbApplication: /bWB/p
pbApplication amp; Usage: /bWestern blotting (0.5-4 micro;g/ml). However, the optimal conditions should be determined individually. The immunoaffinity purified antibody detects a 130 kDa protein, corresponding to the apparent molecular weight of Apaf-1 on SDS-PAGE immunoblots./p
pbStorage Temp.: /b-20 deg;C/p
pbShipping : /bgel pack/p
pbShelf Life: /b12 months/p
pbHandling: /bThe antibody solution should be gently mixed before use./p
pbBrief Background Descriptions: /bDuring apoptosis, Apaf-1 (apoptosis protease activating factor 1) binds to cytochrome c (Apaf-2), a reaction that requires dATP, and this leads to the formation of a large multimeric complex. The complex recruits pro-caspase-9, and activates caspase-9 (Apaf-3). Activated caspase-9 in turn cleaves and activates caspase-3, one of the proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf-1 is ubiquitously expressed in human tissues./p