APAF-1-ALT (H324) Antibody

1 mg/ml in Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2.

Purification & Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity

APAF-1-ALT(H324) antibody detects endogenous levels of APAF-1-ALT protein.

Applications (Recommended Dilutions)

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Background

Studies of apoptosis using the nematode Caenorhabditis elegans as a model system have identified three genes, ced-3, ced-4 and ced-9, as core components in cell death. The Ced-4 protein functions downstream of the apoptotic inhibitor Ced-9 and upstream of Ced-3 to promote cell death. Direct interactions of Ced-4 with Ced-9 and Ced-3 suggest a central role for this protein in the regulation of apoptosis. The mammalian protein Apaf-1 contains a domain with homology to Ced-4 and may function in a similar manner by mediating apoptosis downstream of the survival protein Bcl-2 and upstream of the apoptotic inducing caspases. Apaf-1 has been shown to bind to cytosolic cytochrome c, and it may mediate the activation of caspase-3 by cytochrome c.