Anti-ZNF777 Antibody

Anti-ZNF777 Antibody LS-B3591

ZNF777 Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3591 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3591 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:12500. Learn more

ZNF777 Antibody Details

Target: Human ZNF777

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (0.2 - 1)

Optimal dilution to be determined by the researcher.

 

ZNF777 Antibody Specificity

Immunogen: ZNF777 antibody was raised against synthetic peptide from internal region of human ZNF777 (NP_056509.2). Percent identity by BLAST analysis: Human, Bovine, Rabbit, Horse, Pig, Guinea pig (100%); Dog (92%).

Epitope: Internal

Specificity: Human ZNF777

ZNF777 Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.