Anti-YWHAE / 14-3-3 Epsilon Antibody

Anti-YWHAE / 14-3-3 Epsilon Antibody LS-B3683

YWHAE / 14-3-3 Epsilon Rabbit anti-Sheep Polyclonal (N-Terminus) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3683 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for LS-B3683 was determined to be 1:400. Western Blot: LS-B3683 detects a band/s of approximately 30kD in HEK293 cell lysates. Learn more

YWHAE / 14-3-3 Epsilon Antibody Details

 

 

Target:

Sheep YWHAE / 14-3-3 Epsilon

 

Host:

Rabbit

 

Reactivity:

Bovine, Chicken, Human, Mouse, Rabbit, Rat, Sheep

 

Clonality:

IgG Polyclonal

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (1:400)

 

Western blot (WB) (1:3000)

 

ELISA

 

Optimal dilution to be determined by the researcher.

 

YWHAE / 14-3-3 Epsilon Antibody Specificity

 

Immunogen:

YWHAE / 14-3-3 Epsilon antibody was raised against synthetic peptide corresponding to acetylated N-terminal sequence of sheep 14-3-3 epsilon. ( N-TERMINAL ).

 

Epitope:

N-Terminus

 

Specificity:

Recognizes the acetylated N-terminal of 14-3-3 epsilon. It is a member of the 14-3-3 family which consists of 30 kD proteins that are involved in multiple protein kinase signalling pathways, regulation of cell cycle progression, cytoskeletal structure, transcription, intracellular trafficking and targeting. Protein interactions with 14-3-3 show distinct preference for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein. 14-3-3 epsilon is expressed in all mammals. It is deleted in small cell lung cancers disrupting the G(2) checkpoint. During apoptosis, cleavage of 14-3-3 protein by caspase-3 increases the affinity between bad and Bcl-x(L). When bound by ERK, 14-3-3 epsilon is thought to deactivate heat shock factor 1 in cells recovering from heat shock. It is also a potential suicide susceptibility gene. May not react with recombinant proteins that are not N-acetylated.

YWHAE / 14-3-3 Epsilon Antibody Packaging

 

Presentation:

Serum, 0.09% sodium azide.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.