Anti-Ubiquitin Antibody

Anti-Ubiquitin Antibody LS-B3328

Ubiquitin Mouse anti-Bovine Monoclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3328 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3328 was determined to be 10 ug/ml. Western Blot: 1-3 ug/ml; Prepare lysis buffer in 10 mM N-ethylmaleimide to inhibit ubiquitin-conjugating enzymes. N-ethylmaleimide inactivates certain enzymes by blocking free sulfhydryls. After electrophoresis and transfer, pre-incubate transferred membranes in denaturing buffer (6M guanidine-HCl 20 mM Tris-HCl, pH 7.5, 5 mM b-mercaptoethanol, 1 mM PMSF) for 30-60 minutes at 4uC, followed by extensive PBS washing. Learn more

Ubiquitin Antibody Details

 

 

Target:

Bovine Ubiquitin

 

Host:

Mouse

 

Reactivity:

Bovine, Human, Mouse

 

Clonality:

IgG1,k Monoclonal

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Immunohistochemistry - Frozen (IHC-Fr)

 

Western blot (WB)

 

Immunofluorescence (IF)

 

ELISA (0.1 - 1 µg/ml)

 

Optimal dilution to be determined by the researcher.

 

Ubiquitin Antibody Specificity

 

Immunogen:

Ubiquitin antibody was raised against purified bovine ubiquitin conjugated to carrier protein.

 

Specificity:

Recognizes bovine ubiquitin, both conjugated and unconjugated. Reacts with a single chain 8.5kD protein. The ubiquitin molecule appears to be present in all eukaryotic cells and has an identical primary structure in all animals. Species cross-reactivity: Human, chicken, mouse, C. elegans and Drosophila.

Ubiquitin Antibody Packaging

 

Presentation:

PBS, pH 7.4, 0.1% sodium azide

 

Storage:

Long term: Add glycerol (40-50%) -20°C; Short term: +4°C

 

Restrictions:

For research use only.