Anti-TRPV6
- 貨號 Cat. No.
- ACC-036
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPV6
| Product#: | ACC-036 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)NRGLEDGESWEYQI corresponding to amino acid residues 712-725 of human TRPV6 (Accession Q9H1D0). |
| Putative epitope location: |
Intracellular, C-Terminus. |
 |
| Homology: |
| Rat, mouse-13/14 amino acid residues identical. |
| Reactivity Confirmed: |
| Human, rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of TRPV6 in PC-3 cell line lysate: 1. Anti-TRPV6 antibody (#ACC-036) (1:200). 2. Anti-TRPV6 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of TRPV6 in rat Dorsal Root Ganglion |
 |
Immunohistochemical staining of TRPV6 channel in rat dorsal root ganglion (DRG) using Anti-TRPV6 antibody (#ACC-036) (1:100). A, TRPV6 channel (red) in DRG neurons. B, Staining with mouse anti Parvalbumin (green) in the same DRG section. C, Confocal merge of TRPV6 and Parvalbumin demonstrates existence of the parvalbumin calcium binding protein in a subset of neurons expressing TRPV6 (arrows). |
| Immunocytochemistry: |
| Expression of TRPV6 in PC-3 cells |
 |
| Immunocytochemistry staining of PC-3 cells with Anti-TRPV6 antibody (#ACC-036). Cells were fixed with PFA 4% and then incubated with Anti TRPV6 (1:100) (A), or with a mixture of Anti-TRPV6 (1:100) and TRPV6 Control Peptide (1:20) (B). Alexa Fluor 555 (Red) (1:400) was used as second antibody and Hoechst 33342 for counterstain (blue). |
| References: |
| 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. |
| 2. Clapham, D.E. (2003) Nature 426, 517. |
| 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. |
| 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. |
| 5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183. |
| 6. Hoenderop, J.G.J. et al. (2003) Pflugers Arch 446, 304. |
| 7. Peng, J-B. et al. (2003) J. Physiol. 551, 729. |
| 8. Fixemer, T. et al. (2003) Oncogene 22, 7858. |
| 9. Wissenbach, U. et al. (2001) J. Biol. Chem. 276, 19461. |
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5 TRPV6 (CaT1) and TRPV5 (ECaC) are unique members of the TRP family. Both are epithelial calcium channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV6 and TRPV5 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption. TRPV6 is highly expressed in the placenta, small intestine and kidney.6,7
While not being expressed in benign prostate tissue, TRPV6 was found to be upregulated in prostate cancer tissue and correlation between expression and tumor grade was shown. Finally, TRPV6 was suggested as prognostic marker and as a promising candidate for therapeutic strategies.8,9
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human TRPV6. The Anti-TRPV6 antibody (#ACC-036) can be used for Western Blot analysis, Immunocytochemical and Immunohistochemical applications. It has been designed to recognize TRPV6 from human, rat and mouse samples.