Anti-TRPV5
- 貨號 Cat. No.
- ACC-035
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPV5
| Product#: | ACC-035 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide (C)GLNLSEGDGEEVYHF corresponding to amino acid residues 715-729 of human TRPV5 (Accession Q8NDW5). |
| Putative epitope location: |
intracellular, C-terminus. |
 |
| Homology: |
Rat-11/15 amino acid residues identical; rabbit-13/15 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, human. |
Applications:
| Western Blotting: |
 | Western blotting of TRPV5 in rat kidney (lanes 1 and 3) and PC3 cell line (lanes 2 and 4) lysates: 1,2. Anti-TRPV5 antibody (#ACC-035) (1:200). 3,4. Anti-TRPV5 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of TRPV5 in rat kidney |
 |
Immunohistochemical staining of rat kidney paraffin embedded section using Anti-TRPV5 antibody (#ACC-035) (1:100). TRPV5 is expressed in collecting ducts (CD) in the renal cortex. Note that both proximal tubules (PT) and distal tubules (DT) are negative. Counterstain is Hematoxilin. |
| Immunocytochemistry: |
| . |
 |
| Immunocytochemical staining of PC-3 cells with Anti-TRPV5 antibody (#ACC-035). Cells were fixed with PFA 4% and then incubated with Anti TRPV5 (1:100) (A), or with a mixture of Anti-TRPV5 (1:100) and TRPV5 Control Peptide (1:20) (B). Alexa Fluor 555 (Red) (1:400) was used as second antibody and Hoechst 33342 for counterstain (blue). |
| References: |
| 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. |
| 2. Clapham, D.E. (2003) Nature 426, 517. |
| 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. |
| 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. |
| 5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183. |
| 6. Hoenderop, J.G.J. et al. (2003) Pflugers Arch 446, 304. |
| 7. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729. |
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5 TRPV5 (ECaC) and TRPV6 (CaT1) are unique members of the TRP family. Both are epithelial Ca2+ channels, form constitutively open channels, share high degree of homology (about 66%) with differences in the N and C-terminus. TRPV6 and TRPV5 are expressed in a 1,25-dihydroxyvitamin D3 dependent manner. In contrast to other members of the TRP family, TRPV5 and TRPV6 are highly Ca2+ selective and are expressed in Ca2+ transporting epithelia where they are assumed to have an important role in Ca2+ reabsorption.6,7 TRPV5 is preferentially expressed in kidney.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human TRPV5. The Anti-TRPV5 antibody (#ACC-035) can be used for Western Blot analysis, Immunocytochemical and Immunohistochemical applications. It has been designed to recognize TRPV5 from human and rat samples.