Anti-TRPV4

 

Product#:

ACC-034

Sizes:

50 ml

0.2 ml

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously

expressed in excitable and non-excitable cells.1 According to IUPHAR the TRP family comprises of three main subfamilies on the basis of sequence

homology; TRPC, TRPM and TRPV (to date, three extra subfamilies are considered to belong to the TRP family; the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵ TRPV4 (also named OTRPC4) is activated under hypotonic conditions and serves as an osmoreceptor. TRPV4 is expressed in brain, liver, kidney, heart, testis and salivary gland.⁶

Host:

Rabbit.

Epitope:

Peptide CDGHQQGYAPKWRAEDAPL corresponding to residues 853-871 of Rat TRPV4 (Accession Q9ERZ8).

Putative epitope location:

Intracellular, C-Terminus.

Homology:

Mouse (17/19 residues identical), human (16/19 residues identical).

Reactivity Confirmed:

Rat.

Applications:

Western Blotting:

ND7/23 (mouse neuroblastoma x rat DRG neuron hybrid cell line) lysate (1:200).

Western blotting of ND7/23 cell line lysate: 1. Anti-TRPV4 antibody (#ACC-034) (1:200). 2. Anti-TRPV4 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Rat dorsal root ganglion frozen sections.

Immunohistochemical staining of TRPV4 using Anti-TRPV4 antibody (#ACC-034) in rat dorsal root ganglion (DRG). A, TRPV4 channel (red) in DRG neurons. B, staining with mouse anti Parvalbumin (green) in the same DRG section. C, Confocal merge of TRPV4 and Parvalbumin demonstrates existence of the parvalbumin calcium binding protein in a subset of neurons expressing TRPV4.

Immunocytochemistry:

Expression of TRPV4 in rat dorsal root ganglion (DRG)

Immunocytochemical staining of TRPV4 in rat dorsal root ganglion (DRG) primary culture. Paraformaldehyde-fixed and permeabilized DRG cells were stained as described. A, D. Immunocytochemical staining using Anti-TRPV4 antibody (#ACC-034), (1:500) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. F. Merged images of panels D and E.

References:

1. Montell, C. et al. (2002) Mol. Cell. 9, 229.

2. Clapham, D.E. (2003) Nature 426, 517.

3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.

4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.

5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183.

6. Peng, J.B. et al. (2003) J. Physiol. 551.3, 729.