Anti-TRPV3 (extracellular)
- 貨號 Cat. No.
- ACC-033
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPV3 (extracellular)
| Product#: | ACC-033 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
Rabbit. |
| Epitope: |
Peptide (C)REEEAIPHPLALTHK corresponding to amino acid residues 464-478 of human TRPV3 (Accession Q8NET8). |
 |
| Putative epitope location: |
| 1st extracellular loop. |
| Homology: |
Rat-13/15 amino acid residues identical, Mouse ?12/15 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat DRG (lanes 1 and 2) and rat brain (lanes 3 and 4) lysates: 1, 3. Anti-TRPV3 (extracellular) antibody (#ACC-033) (1:200). 2, 4. Anti-TRPV3 (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of TRPV3 channel in mouse spinal cord |
 |
Immunohistochemical staining of TRPV3 in mouse spinal cord using Anti-TRPV3 (extracellular) antibody (#ACC-033). A, TRPV3 (green) appears in neurons (vertical arrows) in the ventral horn of the mouse spinal cord. B, motor neurons were stained with goat anti choline-acetyltransferase (red). In some motor neurons (A) TRPV3-extra was more intense (vertical arrow) whereas in others it was weak (horizontal arrows). C, Merged images of panels A and B. The inset in C magnifies one large motor neuron (vertical arrowhead). Note punctate pattern of TRPV3-extra on the surface of the soma. |
| Immunocytochemistry: |
Immunocytochemical staining of TRPV3 channel in rat DRG primary culture. |
 |
A. Rat DRG primary culture stained with Anti-TRPV3 (extracellular) antibody (#ACC-033) (1:50-1:100), followed by goat-anti-rabbit-AlexaFluor-555 secondary antibody. B. Nuclear staining of rat DRG primary culture cells with the cell permeable dye Hoechst 33342. C. Merged images of panels A and B. |
| References: |
| 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. |
| 2. Clapham, D.E. (2003) Nature 426, 517. |
| 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. |
| 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. |
| 5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183. |
| 6. Tominaga, M. and Caterina, M.J. (2004) J.Neurobiol. 61, 3. |
| 7. Voets, T. et al. (2004) Nature 430, 748. |
| 8. Xiao, R. et al. (2008) J.Biol.Chem. 283, 6162. |
TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels havesixputative transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.3
According to IUPHAR, the TRP family is composed of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).1-4 The TRPV subfamily consists of six members, TRPV1-6.5
Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17èš“) to noxious heat (>52èš“).6,7
TRPV3 is highly expressed in skin keratinocytes. It forms a nonselective cation channel that is activated by hot temperatures in the innocuous and noxious range. The channel is also expressed in oral and nasal cavities where it has been postulated to play a role in flavor sensation. TRPV3 is also implicated as a molecular target of some allergens and skin sensitizers.8
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human TRPV3. The Anti-TRPV3 (extracellular) (#ACC-033) can be used for Western blot analysis, immunohistochemical and immunocytochemical applications. It has been designed to recognize TRPV3 from human, rat and mouse samples.