Anti-TRPV2

貨號 Cat. No.: ACC-032
規格 Size: 50 ml 0.2ml

Description

Anti-TRPV2

Product#:

ACC-032

Sizes:

50 ml

0.2 ml

Epitope:

Peptide (C)KKNPTSKPGKNSASEE corresponding to residues 735-750

of rat TRPV2 (Accession Q9WUD2).

Putative epitope location:

C-Terminus, Intracellular.

Homology:

Mouse ?identical.

Reactivity Confirmed:

Rat.

Applications:

Western Blotting:

Western blotting of rat brain membrane (1,2) and RBL lysates (3,4):

1,3. Anti-TRPV2 antibody, 1:200.

2,4. Anti-TRPV2 antibody, preincubated with the control peptide antigen.

Immunoprecipitation:

Immunoprecipitation of rat basophilic leukemia (RBL) lysate:

1. RBL lysate.

2. RBL lysate immunoprecipitated with Anti-TRPV2 antibody (#ACC-032) (6 mg).

3. RBL lysate immunoprecipitated with pre-immune rabbit serum.

The upper arrow indicates the TRPV2 channel while the lower arrow indicates the IgG heavy chain.

Western blotting was performed with Anti-TRPV2 antibody.

References:

1. Montell, C. et al. (2002) Mol. Cell. 9, 229.

2. Clapham, D.E. (2003) Nature 426, 517.

3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362.

4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591.

5. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183.

6. Tominaga, M. and Caterina, M.J. (2004) J.Neurobiol. 61, 3.

7. Voets, T. et al. (2004) Nature 430, 748.

8. Pedersen, S.F. et al. (2005) Cell Calcium 38, 233.

9. Muraki, K. et al. (2003) Circ. Res. 93, 829.

10. Shimosato, G. et al. (2005) Pain 119, 225.

11. Saunders, C.I. et al. (2007) Mol. Immunol. 44, 1429.

12. Caterina, M.J. et al. (1999) Nature 398, 436.

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹ The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.³

According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵

Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17èš“) to noxious heat (>52èš“).^6,7 Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52èš“ and is considered to play an essential role in the perception of high-intensity noxious heat stimulation.^8-10The TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway.⁸ TRPV2 channel is expressed in DRG neurons, different brain region and non-neuronal tissues such as spleen, lung and, intestine and in component of the immune system.^5,11-12

Alomone Labs is pleased to release two novel antibodies to both intracellular TRPV2 and extracellular TRPV2, Anti-TRPV2 (#ACC-032) and Anti-TRPV2 (extracellular) (#ACC-039), respectively.

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