Anti-TRPV2 (extracellular)-FITC

Anti-TRPV2 (extracellular)-FITC

Western blotting of  ND7/23 cell line membrane (1,3), RBL lysates (2,4) and rat brain membrane (5,6) using the uncojugated antibody: 1,2,5. Anti-TRPV2 (extracellular) antibody (ACC-039), (1:200). 3,4,6. Anti-TRPV2 (extracellular) antibody, preincubated with the control peptide antigen.

Direct flow cytometry analysis of intact living RBL cells: _____:  unstained cells. _____: Anti-TRPV2 (extracellular)-FITC antibody, (#ACC-039-F).

TRP channels are a large family (about 28 genes) of plasma membrane, non-selective cationic channels that are either specifically or ubiquitously expressed in excitable and non-excitable cells.¹ The TRP channels have putative six-transmembrane domains (TM) with a pore domain between the fifth and the sixth TM, and all assemble as tetramers. Both the N- and the C-terminus of all TRPs are intracellular.³

According to IUPHAR, the TRP family is comprised of three main subfamilies on the basis of sequence homology; TRPC, TRPM and TRPV (to date, three additional subfamilies are also considered to belong to the TRP family: the TRPA, TRPML, and TRPP).^1-4 The TRPV subfamily consists of six members, TRPV1-6.⁵

Four members of the TRPV family have been described as a thermosensitive ion channels (TRPV1 to TRPV4). Each channel exhibits distinct thermal activation thresholds ranging from noxious cold (<17èš“) to noxious heat (>52èš“).^6,7 Although it shares around 50% homology with TRPV1, TRPV2 is not activated by capsaicin nor by protons. It has a high temperature threshold of ~52èš“ and is considered to play an essential role in the perception of high-intensity noxious heat stimulation.^8-10 The TRPV2 is also considered to be a stretch-activated channel and to play a role in skeletal and cardiac muscle degeneration and pain pathway.⁸ TRPV2 channel is expressed in DRG neurons, different brain region and non-neuronal tissues such as spleen, lung and, intestine and in component of the immune system.^5,11-12

Alomone Labs is pleased to offer a new version of the Anti-TRPV2 (extracellular) antibody (#ACC-039) directly conjugated to FITC. The Anti-TRPV2 (extracellular)-FITC antibody (#ACC-039-F) can be used for immunofluorescent applications such as direct flow cytometry and was design to  recognizes TRPV2 from rat and mouse samples.