Anti-TRPV1
- 貨號 Cat. No.
- ACC-030
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPV1
(VR1)
| Product#: | ACC-030 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
| Peptide (C)EDAEVFKDSMVPGEK corresponding to residues 824-838 of rat TRPV1. (Accession O35433). |
| Putative epitope location: |
| Intracellular, C-terminus. |
 |
| Homology: |
| Guinea pig (14/15 residues identical), Human (10/15 residues identical). |
| Reactivity Confirmed: |
| Human, rat and mouse. |
Applications:
| Western Blotting: |
 | Western blotting of TRPV1 transfected HEK-293 cells: 1. Anti-TRPV1 antibody (#ACC-030) (1:200). 2. Anti-TRPV1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
(Above) Staining of TRPV1 in kidney using Anti-TRPV1 antibody (#ACC-030). Selective subsets of cells were stained in mouse (A) compared to rat (B). Diaminobenzidine color product is blue-black (arrows). The fluorescent counterstain is DAPI (silver). |
 |
| Immunohistochemical staining of TRPV1 using Anti-TRPV1 antibody (#ACC-030) in rat dorsal root ganglion (DRG). A, TRPV1 channel (red) in DRG neurons. B, staining with mouse anti-Parvalbumin (green) in the same DRG section. C, Confocal merge of TRPV1 and Parvalbumin demonstrates the existence of the parvalbumin calcium binding protein in a subset of neurons expressing TRPV1 (arrows). |
| Immunocytochemistry: |
Expression of TRPV1 in rat dorsal root ganglion (DRG) primary culture |
 |
Immunocytochemical staining of TRPV1 in rat DRG. Paraformaldehyde-fixed and permeabilized DRG cells were stained as described. A, Immunocytochemical staining using Anti-TRPV1 antibody (#ACC-030), (1:200) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B, Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B. |
| References: |
| 1. Gunthorpe, M.J.et al. (2002) Trends. Pharmacol. Sci. 23, 183. |
| 2. Cesare, P. et al. (1999) J Proc Natl Acad Sci. 96, 7658. |
| 3. Kim, J. et al. (2003) Nature 424, 81. |
| 4. Ahern, G. P. et al. (2003) J. Biol. Chem. 278, 30429. |
| 5. Ross, R.A. et al. (2003) Br. J. Pharmacol. 140, 790. |
| 6. Tominaga, M. et al. (1998) Neuron 21, 531. |
| 7. Vlachova, V. et al. (2003) J. Neurosci. 23, 1340. |
| 8. Agopyan, N. et al. (2004) Am. J. Physiol .Lung .Cell .Mol. Physiol. 286, 563. |
| 9. Reilly, C.A. et al. (2003) Toxicol. Sci. 73, 170. |
TRPV1 (also named VR1, capsaicin receptor and vanilloid receptor) is a member of the transient receptor potential (TRP) channel family, which includes
TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.
TRPC, TRPM, TRPA, TRPP, TRPML and the TRPV subfamilies. The TRPV subfamily consists of six members named, TRPV1-6. The TRPV1 channel is a vanilloid gated, nonselective cation channel.
The channel has sequence homology to the TRP family, and shares a similar predicted structure of six transmembrane domain (TM) with a pore loop
between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3
between TM5 and TM6.1 TRPV1 is expressed predominantly in nociceptors and in sensory neurons.2,3
TRPV1 has many activators among them heat, protons, vanilloids like capsaicin, reiniferatoxin (RTX), and lipids. This channel is associated with tissue injury and inflammation.5,6
Other members of this family, TRPV2, TRPV3 and TRPV4 also show thermal sensitivity.7 Conformational changes in the C-terminus are responsible for many
functions such as permeation and gating.
functions such as permeation and gating.
There is also evidence that deletion of the C-terminus causes a loss of sensitivity to any stimuli.7 Recent studies demonstrated involvement of TRPV1 in apoptosis where inhibition of the receptor prevented apoptosis.8,9