Anti-TRPM8 (extracellular)
- 貨號 Cat. No.
- ACC-049
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPM8 (extracellular)
| Product#: | ACC-049 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
| Peptide SDVD GTTYDFAHC corresponding to amino acid residues 917-929 of human TRPM8 (Accession Q7Z2W7). |
 |
| Putative epitope location: |
3rd extracellular loop. |
| Homology: |
Canis ?identical, bovine ?12/13, rat and mouse ?11/13 amino acid residues identical. |
| Reactivity Confirmed: |
| Human, mouse, rat. |
Applications:
| Western Blotting: |
 | Western blotting of prostate carcinoma cell lines; DU145 (lanes 1,3), LNCaP (lanes 2,4) and mouse-TRPM8 transfected HEK-293 (lanes 5,6) cell lines lysates : 1,2,5. Anti-TRPM8 (extracellular) antibody (#ACC-049) (1:200). 3,4,6. Anti-TRPM8 (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of TRPM8 in dorsal root ganglia (DRG) |
 |
Immunohistochemical staining of TRPM8 in rat DRG frozen section using Anti-TRPM8 (extracellular) antibody (#ACC-049), (1:100). TRPM8 is expressed in DRG neurons. Counterstain is Hoechst 33342 |
| Immunocytochemistry: |
Expression of TRPM8 in the prostate carcinoma cell line, LNCaP. |
 |
Immunocytochemical staining of LNCaP cells with Anti-TRPM8 (extracellular) antibody (#ACC-049). A, Extracellular staining of intact LNCaP cells, incubated with Anti-TRPM8 (extracellular) antibody (1:100), followed by goat-anti-rabbit-Alexa fluor-550 (red) (x100). B, Intracellular staining of LNCaP cells incubated with Anti-TRPM8 (extracellular) antibody (1:1000), followed by goat-anti-rabbit-Alexa fluor-488 (green) (x100). Expression of TRPM in root dorsal root ganglion (DRG) cells |
 |
Immunocytochemical staining of TRPM8 in root dorsal root ganglion (DRG) cells. A. Intracellular staining of Paraformaldehyde-fixed and permeabilized DRG cells with Anti-TRPM8 (extracellular) antibody (#ACC-049), (1:500) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Extracellular staining of live DRG cells with Anti-TRPM8 (extracellular) antibody (#ACC-049), (1:50) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. The cell-permeable dye Hoechst 33342 (blue) was used for nuclear staining. |
| References: |
| 1. Fleig, A. and Penner, R. (2004) Trends Pharmacol. Sci. 25, 633. |
| 2. Brauchi, S. et al. (2004) Proc. Natl. Acad. Sci. 101, 15494. |
| 3. McKemy, D.D. et al. (2002) Nature416, 52. |
| 4. Peier, A.M. et al. (2002) Cell 108, 705. |
| 5. Dragoni, I. et al. (2006) J.Biol.Chem. 281, 37353. |
| 6. Zhang, L. and Barritt, G.J. (2006) Endocr. Relat. Cancer13, 27. |
The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first member, melastatin (TRPM1), whose gene was identified in metastatic and benign melanomas.1 The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N-terminus and C-terminus. However, due to their long N-terminus and C-terminus they were also named the long TRP channel family.1
The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7.1
The TRPM8 is the cold and menthol receptor activated by either cold temperatures(~28èš“) or menthol.2
TRPM8 is expressed in dorsal root ganglia (DRGs) where about 5%-10% of the small diameter DRG neurons express the channel.3,4 In DRGs, TRPM8 expressing neurons do not express the TRPV1 channel.5
Overexpression of TRPM8 was found in prostate cancer cells. However, the physiological and pathological roles that these cells play is still elusive.6 In prostate, it was suggested that TRPM8 might play a possible role in the progression of cancer to the metastatic stage.6