Anti-TRPM4

Anti-TRPM4

Western blot analysis of TRPM4 in rat lung (lanes 1 and 5), rat heart membrane (lanes 2 and 6), mouse brain membrane (lanes 3 and 7) and ms1 cells lysate (lanes 4 and 8): 1-4. Anti-TRPM4 antibody (#ACC-044), (1:200). 5-8.  Anti-TRPM4 antibody, preincubated with the control peptide antigen.

Immunohistochemical staining of rat lung paraffin embedded sections using Anti-TRPM4 antibody (#ACC-044), (1:100). TRPM4 is expressed in the respiratory epithelium of the bronchioli (arrows). Hematoxilin is used as the counterstain.

The mammalian melastatin-related transient receptor potential (TRPM) is a subfamily of the TRP family. The family was named after the first discovered member, melastatin (TRPM1) whose gene was identified in metastatic and benign melanomas^1-3. 

The TRPM family consists of eight members designated as TRPM1-8 that can be further divided into four pairs: TRPM1 and TRPM3; TRPM2 and TRPM8; TRPM4 and TRPM5; and TRPM6 and TRPM7^1,2.

The TRPM proteins share structural homology with other members of the TRP superfamily channels; six putative transmembrane domains, and cytoplasmic N- and C-termini. However, due to their long N- and C-termini they are also named the long TRP channel family^1-3. 

TRPM4 and TRPM5 are the only TRP channels that are permeable to monovalent cations but not to Ca²⁺.

TRPM4 is expressed as at least two alternative spliced isoforms, TRPM4a and TRPM4b which consist of shorter and longer N-terminal regions, respectively. TRPM4a displays little activity, TRPM4b and TRPM5 appear to be directly activated by an increase in intracellular Ca²⁺ concentration⁴.

TRPM4 is expressed at relatively high levels in cardiac tissues.

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the human TRPM4. The Anti-TRPM4 antibody (#ACC-044) can be used for western blot and immunohistochemical applications. It has been designed to recognize TRPM4 from human, rat and mouse samples.