Anti-TRPC6
- 貨號 Cat. No.
- ACC-017
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPC6
| Product#: | ACC-017 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
| Peptide (C)RRNE SQDYL LMDEL G, corresponding to residues 24-38 of mouse TRPC6 (Accession Q61143). |
| Putative epitope location: |
Intracellular, N-terminus. |
| Homology: |
Rat - identical, human -15/16 residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat brain membranes: 1. 2. Anti-TRPC6 antibody, preincubated with the control peptide antigen. Anti-TRPC6 antibody (#ACC-017) (1:200). |
| Immunohistochemistry: |
| Mouse cerebellum frozen sections. |
 |
Immunohistochemical staining of TRPC6 using Anti-TRPC6 antibody (#ACC-017) in mouse cerebellum. A, TRPC6 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) layer. B, staining with mouse anti parvalbumin in the same brain section. C, Confocal merge of TRPC6 and PV demonstrates partial colocalization in the Purkinje and the molecular layers. |
| References: |
| 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. |
| 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. |
| 3. Clapham, D.E. (2003) Nature 426, 517. |
| 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. |
| 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. |
| 6. Winn. M.P. et al. (2005) Science, 308, 1801. |
| 7. Reiser, J. et al. (2005) Nat. Genet. 37, 739. |
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals, about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV (vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4
The TRPC subfamily consists of seven proteins named TRPC1 to 7 which can be further divided into four subgroups based on their sequence homology and functional similarities: 1. the TRPC1 2. TRPC4 and TRPC5 3. TRPC3, TRPC6, TRPC7 4. TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in peripheral tissues.
TRPC6 can form heterotetramers with TRPC3 and TRPC7. It is primarily expressed in brain, lung and muscle. High levels of expression of the channel were also found in human platelets. Recently it was reported that TRPC6 is also expressed in the kidney where a mutated channel has been implicated in kidney failure disease.6,7