Anti-TRPC1
- 貨號 Cat. No.
- ACC-010
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPC1
| Product#: | ACC-010 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide QLYDK GYTSK EQKDC, corresponding to amino acid residues 557-571 of human TRPC1 (Accession P48995). |
| Putative epitope location: |
Intracellular. |
 |
| Homology: |
Mouse, rat, human - identical; rabbit, bovine - 14/15 amino acid residues identical; Xenopus - 11/14 amino acid residues identical. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain membranes. 1. Anti-TRPC1 antibody (#ACC-010), (1:200). 2. Anti-TRPC1 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of TRPC1 in mouse cerebellum |
 |
Immunohistochemical staining of TRPC1 using Anti-TRPC1 antibody (#ACC-010) in mouse cerebellum frozen section. A, TRPC1 (red) appears in Purkinje cells (arrows) and in the molecular (Mol) and granule (Gran) layers. B, staining with mouse anti parvalbumin (PV) in the same brain section. C, Confocal merge of TRPC1 and PV demonstrates partial co-localization in the Purkinje and the molecular layers. |
| References: |
| 1. Moran, M.M. et al. (2004) Current Opin. Neurobiol. 14, 362. |
| 2. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. |
| 3. Clapham, D.E. (2003) Nature 426, 517. |
| 4. Padinjat, R. and Andrews, S. (2004) J. Cell. Sci. 117, 5707. |
| 5. Huang, C.L. (2004) J. Am. Soc. Nephrol. 15, 1690. |
| 6. Liu, X. et al. (2003) J. Biol.Chem. 278, 11337 |
The Transient Receptor Potential (TRP) superfamily is one of the largest ion channel families and consists of diverse groups of proteins. In mammals
about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV
(vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4
about 28 genes encode the TRP ion channel subunits. The mammalian TRP superfamily comprises six subfamilies known as the TRPC (canonical), TRPV
(vanilloid), TRPM (melastatin), TRPML (mucolipins), TRPP (polycystin) and the TRPA (ANKTM1) ion channels.1-4
The TRPC subfamily consists of seven proteins named TRPC1 to 7, which can be further divided into four subgroups based on their sequence homology and
functional similarities:
functional similarities:
1.) The TRPC1 2.) TRPC4 and TRPC5 3.) TRPC3, TRPC6, TRPC7 4.) TRPC2.2,5 They are highly expressed in the central nervous system and to a lesser extent in
peripheral tissues.
peripheral tissues.
TRPC1 was the first mammalian TRP protein that was reported to form an ion channel.2 It can co-assemble with other TRPC subunits (TRPC3, TRPC4, TRPC5)
to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established
as yet.1-3
to form heterotetramers whose properties are distinct from that of their homomeric form. The existence of the TRPC1 homomers has not been established
as yet.1-3
The TRPC1, TRPC4 and TRPC5 can be activated either by Ca2+ store depletion or by GPCR stimulation pathways, while TRPC3, TRPC6 and TRPC7 form
non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated
channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells.6
non-selective cationic channels that are activated by the stimulation of GPCRs. TRPC1, 4 and TRPC5 are assumed to form components of store operated
channels in some cell types such as salivary gland cells, endothelial cells and vascular smooth muscle cells.6