Anti-TRPA1 (extracellular)
- 貨號 Cat. No.
- ACC-037
- 規格 Size
- 50 μl 0.2ml
Description
Anti-TRPA1 (extracellular)
| Product#: | ACC-037 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
| Peptide (C)NSTGIINETSDHSE corresponding to 747-760 residues of human TRPA1 (Accession O75762). |
 |
| Putative epitope location: |
1ST extracellular loop. |
| Homology: |
Bovine-identical, canis-13/14 residues identical, rat- 12/14/ residues identical, mouse-9/14 residues identical. |
| Reactivity Confirmed: |
| Rat and mouse. |
Applications:
| Western Blotting: |
Expression of TRPA1 in rat DRG and PC12 (adrenal gland pheochromocytoma) cells |
 | Western blotting of rat DRG (lanes 1,2), non-differentiated PC12 cells (lanes 3,5) and differentiated PC12 cells (lanes 4,6) lysates: 1,3,4. Anti-TRPA1 (extracellular) antibody (#ACC-037) (1:200). 2,5,6. Anti-TRPA1 (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
| Expression of TRPA1 in mouse dorsal root ganglia frozen section. |
 |
Immunohistochemical Staining of TRPA1 using Anti-TRPA1 (extracellular) antibody (#ACC-037) in mouse dorsal root ganglion (DRG). A, TRPA1 (green) was distributed in patches (horizontal arrows). B, Neurons containing neurofilament 200 (red) also were distributed in patches. C, Confocal merge of TRPA1 and neurofilament 200 demonstrate partial overlap of these patches (arrows). |
| References: |
| 1. Kerstin Hill and Michael Schaefer (2007) J. Biol. Chem. 282, 7145. |
| 2. story, G.M. et al. (2003) Cell 112, 819. |
| 3. Bandell, M. et al. (2004) Neuron 41, 849. |
| 4. Jordt, S.E. et al. (2004) Nature 427, 260. |
| 5. Zurborg, S. et al. (2007) Nat Neurosci, 10, 277. |
| 6. Corey, D. P. et al. (2004) Nature 432, 723. |
| 7. Sotomayor, M.,et al. (2005) Structure 13, 669. |
| 8. Clapham, D.E. (2002) Science 295, 2228. |
The TRPA family is comprised of only one mammalian member, the TRPA1 (formerly named ANKTM1). TRPA1 is expressed in peripheral sensory neurons, where it is suggested to contribute to the detection of painful stimuli.1
Originally it was thought that TRPA channels sensed painfully cold temperatures2, but a more conservative description is that TRPA1 is sensitive to membrane/cytoskeletal perturbations by cold3-5 and perhaps stretch.6 In addition, it is sensitive to pungent natural compounds present in cinnamon oil, mustard oil, and wintergreen oil.
TRPA1 is also expressed in hair cells, where its role in sensing mechanical forces is still unclear and controversial.1
TRPA1 has similar structure as all TRP ion channel members, six transmembrane domains, intracellular N-and C-terminus. However, its N-terminus domain possesses 17 ankyrin repeats that might indicate its potential role as a mechanosensor.6,7
TRPA1 is expressed in nociceptive neurons expressing TRPV1 as well and might serve as a marker for poly-modal nociceptors.8
Alomone Labs offers Anti-TRPA1 antibody directed against an extracellular epitope of the human TRPA1.