Anti-TP53 / p53 Antibody

Anti-TP53 / p53 Antibody LS-B431

TP53 / p53 Mouse anti-Human Monoclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B431 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B431 was determined to be 2.5 ug/ml. Learn more

TP53 / p53 Antibody Details

 

 

Target:

Human TP53 / p53

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG2a,k Monoclonal

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (2.5)

 

Western blot (WB) (1:500 - 1:2000)

 

Immunoprecipitation (IP)

 

ELISA (1:2000 - 1:10000)

 

Optimal dilution to be determined by the researcher.

 

TP53 / p53 Antibody Specificity

 

Immunogen:

TP53 / p53 antibody was raised against recombinant human TP53 / p53.

 

Specificity:

Recombinant human p53 protein. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse myeloma P3-X63/AG8.653 cells using conventional hybridoma technology.

TP53 / p53 Antibody Packaging

 

Presentation:

0.02 M potassium phosphate, 0.5 M sodium chloride, pH 7.2, 0.01% sodium azide.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.