Anti-TGF-Beta Antibody

Anti-TGF-Beta Antibody LS-B4772

TGF-Beta Mouse anti-Human Monoclonal (TB21) Antibody

An IHC-plusTM AntibodySuitable for use in Western Blot, ELISA, Neutralization and Immunohistochemistry. Neutralization: Neutralizes TGF-beta activity in vitro in an inhibition assay of CCL/64 cell growth and the growth promoting action of TGF-beta in the NRK-49F colony forming assay. The effect of microinjection of this antibody into one blastomere of two cell stage Xenopus embryos indicated that it was able to neutralize effectively the bioactivity of TGF-beta in vivo. Immunohistochemistry (formalin-fixed, paraffin-embedded, frozen): 1:1000. Has been used to demonstrate TGF-beta in ovine ovarian tissue and human breast carcinoma. As a consequence of the intense staining of the erythrocytes it is possible to locate a single cell within the ovarian stroma making it useful in locating very fine capillary networks within tissue. Western Blot: 5-20 ng/ml will allow visualization of 100 ng/lane of TGF-beta. Recognizes the dimeric (25kD) and monomeric (12.5kD) forms of TGF-beta under both non-reducing and reducing conditions respectively. Learn more

TGF-Beta Antibody Details

Target: Human TGF-Beta

Host: Mouse

Reactivity: Human

Clonality: IgG1,k Monoclonal [TB21] 

Purification: Protein G Column

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (10)

Immunohistochemistry - Frozen (IHC-Fr)

Western blot (WB)

ELISA

Functional Assay (Func)

Neutralization (Neut)

Optimal dilution to be determined by the researcher.

 

TGF-Beta Antibody Specificity

Immunogen: TGF-Beta antibody was raised against tGF-b from human platelets.

Specificity: Recognizes both human platelet-derived and recombinant TGF-beta.

TGF-Beta Antibody Packaging

Presentation: PBS, pH 7.0.

Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Restrictions: For research use only.