Anti-STIM2
- 貨號 Cat. No.
- ACC-064
- 規格 Size
- 50 μl 0.2ml
Description
Anti-STIM2
| Product#: | ACC-064 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide SAEKQWEVPDTASEC corresponding to amino acid residues 583-597 of human STIM2 (Accession Q9P246). |
 |
| Putative epitope location: |
C-terminus, Intracellular.
|
| Homology: |
Rat, mouse and bovine-identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain (lanes 1,3) and RBL cell line (lanes 2,4) lysates: 1. Anti-STIM2 antibody (#ACC-064), (1:200). 2. Anti-STIM2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of STIM 2 in rat lung. |
 |
Immunohistochemical staining of rat lung paraffin embedded sections using Anti-STIM2 antibody (#ACC-064) (1:100). Staining is present in the respiratory epithelium of the bronchiole (red arrows) as well as in the pneumonocytes of the alveolar wall (blue arrows). Color reaction was obtained with SuperPicture HRP-conjugated polymer (Zymed) followed by DAB. Counterstain is Hematoxilin.
|
| Immunocytochemistry: |
| Expression of STIM2 on rat basophilic leukemia cells. |
 |
Immunocytochemical staining of rat basophilic leukemia (RBL) cells. A, D. A paraformaldehyde-fixed and permeabilized RBL cells were stained with Anti-STIM2 antibody (#ACC-064) (1:100) followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. B, E. Nuclear fluorescence staining of cells using the membrane-permeable DNA dye Hoechst 33342. C. Merged images of panels A and B F. Merged images of panels D and E. |
Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become the focus of intense research.
Recently, several key players of the store-operated complex have been identified: the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2.3 Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4
While STIM1 appears to be localized intracellularly at the ER membrane and at a much lower extent on the cell surface of several cell types, STIM2 appears to be localized solely at the ER membrane.5 The function of STIM2 still remains elusive and several models have been suggested.6-8
Alomone Labs is pleased to offer a highly specific antibody directed against human STIM2. The Anti-STIM2 antibody (#ACC-064) can be used in Western blot, immunocytochemical, immunohistochemical applications. It has been designed to recognize STIM2 from human, rat, mouse and bovine samples.