Anti-STIM1 (extracellular)
- 貨號 Cat. No.
- ACC-063
- 規格 Size
- 50 μl 0.2ml
Description
Anti-STIM1 (extracellular)
| Product#: | ACC-063 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: |
Rabbit. |
| Epitope: |
Peptide CHSED EKLSF EAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession Q13586). |
 |
| Putative epitope location: |
STIM1 expressed on the plasma membrane: Extracellular, N-terminus. STIM1 expressed on the ER: Intracellular, facing the lumen, N-terminus. |
| Homology: |
Rat, mouse, bovine, and canis - identical. |
| Reactivity Confirmed: |
| Human, rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of RBL (rat basophilic leukemia) (lanes 1 and 4), HL-60 (human promyelocytic leukemia) (lanes 2 and 5), and Jurkat (human human lymphoblastic T cell) (lanes 3 and 6) cell line lysates: 1, 2, 3.Anti-STIM1 (extracellular) antibody (#ACC-063), (1:1000). 4, 5, 6. Anti-STIM1 (extracellular) antibody preincubated with the control peptide antigen. |
| Immunocytochemistry: |
Expression of STIM1 in rat RBL cells. |
 |
Immunocytochemical staining of STIM1 in live RBL cells A. Extracellular staining of cells with Anti-STIM1 (extracellular) antibody (#ACC-063), (1:50) followed by Alexa-555-conjugated goat-anti-rabbit secondary antibody. B. Nuclear staining of cells using the cell-permeable dye Hoechst 33342. C. Merged images of A and B. |
| Flow Cytometry: |
 |
Indirect flow cytometry analysis of intact RBL (A) and Jurkat (B) cells. _______ - Cells + FITC-conjugated goat anti-rabbit antibody. _______ - Cells + Anti-STIM1 (extracellular) antibody (#ACC-063), (5-10 mg antibody/0.5-1x106 cells) + FITC-conjugated goat anti-rabbit antibody. |
| References: |
| 1. Eisner, D.A. et al. (2005) Exp. Physiol. 90, 3. |
| 2. Chakrabarti, R. and Chakrabarti, R. (2006) J. Cell. Biochem. 99, 1503. |
| 3. Feske, S. et al. (2006) Nature 441, 179. |
| 4. Pickett, J. (2006) Nat. Rev. Mol. Cell Biol.7,794. |
| 5. Dziadek, M.A. and Johnstone, L.S. (2007) Cell Calcium 42, 123. |
| 6. Yuan, J.P. et al. (2007) Nat. Cell Biol. 9, 636. |
| 7. Muik, M. et al. (2008) J. Biol. Chem.Jan 10 [Epub ahead of print]. |
| 8. Li, Z. et al (2007) J. Biol. Chem.282, 29448. |
| 9. Hewavitharana, T. et al. (2007) Cell Calcium 42, 173. |
Cytosolic calcium (Ca2+) has long been known to act as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, and cell growth and proliferation.1,2 The mechanism controlling the influx of intracellular Ca2+ either by calcium-release-activated Ca2+ channels (CRAC) or from intracellular stores has lately become of great interest.
Recently, several key players of the store-operated complex have been identified.3 the Orai family consisting of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+ sensor.3,4 The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5 STIM1 has an amino-terminal EF hand Ca2+ -binding domain facing the lumen of the ER.6 Upon Ca2+ store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs. Several possible interactions between STIM1 and Orai1 have been suggested. The most simple and cited is a dynamic interaction between the cytosolic C-terminus of STIM1 and the cytoplasmic domain of the Orai1 channel.7-9 STIM1 is assumed to regulate the activity of all known SOCs, including native SOCs.5 Consistent with their important role as calcium sensors within the ER, STIM1 proteins are ubiquitously expressed.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of human STIM1. The Anti-STIM1 (extracellular) antibody (#ACC-063) can be used in Western blot, immunocytochemical, immunohistochemical, and indirect flow cytometry applications. The Anti-STIM1 (extracellular) antibody should recognize STIM1 from human, rat, mouse, bovine, and canis samples.