Anti-STAT5B Antibody

Anti-STAT5B Antibody LS-B4002

STAT5B Rabbit anti-Human Polyclonal (N-Terminus) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4002 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4002 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. Learn more

STAT5B Antibody Details

Target: Human STAT5B

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (1)

Optimal dilution to be determined by the researcher.

 

STAT5B Antibody Specificity

Immunogen: STAT5B antibody was raised against synthetic peptide from N-terminus of human STAT5B (NP_036580.2, P51692). Percent identity by BLAST analysis: Human, Dog, Rabbit, Horse, Pig, Chicken (100%); Bovine (91%); Xenopus (85%).

Epitope: N-Terminus

Specificity: Human STAT5b

STAT5B Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.