Anti-Sphingosine 1-Phosphate Receptor 2
- 貨號 Cat. No.
- ASR-012
- 規格 Size
- 50 µl 0.2 ml
Description
Anti-Sphingosine 1-Phosphate Receptor 2
| Product#: | ASR-012 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
Polyclonal. |
| Epitope: |
Peptide ERQVALAKVKLYGSDKSC, corresponding to amino acid residues 129-146 of mouse Sphingosine 1-Phosphate Receptor 2 (S1PR2), (Accession P52592). |
 |
| Putative epitope location: |
2nd intracellular loop. |
| Homology: |
Rat - 17/18 amino acid residues identical; human - 16/18 amino acid residues identical. |
| Reactivity Confirmed: |
| Rat, mouse, human. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat kidney (lanes 1 and 3) and rat heart (lanes 2 and 4) lysates: 1, 2. Anti-Sphingosine 1-Phosphate Receptor 2 antibody (#ASR-012), (1:200). 3, 4. Anti-Sphingosine 1-Phosphate Receptor 2 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Sphingosine 1-Phosphate Receptor 2 in rat lung |
 |
Immunohistochemical staining of paraffin embedded rat lung sections using Anti-Sphingosine 1-Phosphate Receptor 2 antibody (#ASR-012), (1:100). Sphingosine 1-Phosphate Receptor 2 is expressed in respiratory epithelium (red arrows). Hematoxilin is used as the counterstain. |
Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (SIP), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.
Sphingosine 1-Phosphate Receptor 2 (S1PR2) is ubiquitously expressed. S1PR2 mRNA was detected in the brain, heart, spleen, liver, lung, thymus, kidney and skeletal muscle4. Unlike S1PR1, knockout of SIPR2 gene is not lethal5.
Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the mouse Sphingosine 1-Phosphate Receptor 2. Anti-Sphingosine 1-Phosphate Receptor 2 antibody (#ASR-012) can be used for western blot and immunohistochemistry applications. It has been designed to recognize Sphingosine 1-Phosphate Receptor 2 from mouse, rat and human samples.