Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular)
- 貨號 Cat. No.
- ASR-011
- 規格 Size
- 50 µl 0.2 ml
Description
Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular)
| Product#: | ASR-011 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide SDYVNYDIIVRHYN(C), corresponding to amino acid residues 17-30 of human Sphingosine 1-Phosphate Receptor 1 (S1PR1), (Accession P21453). |
 |
| Putative epitope location: |
Extracellular, N-terminus.
|
| Homology: |
Mouse and rat - 13/14 amino acid residues identical. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of RAEC cells (lanes 1 and 3) and in A-10 (lane 2 and 4) cell lysates: 1, 2. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (#ASR-011), (1:200). 3, 4. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of Sphingosine 1-Phosphate Receptor 1 in rat lung |
 |
| Immunohistochemical staining of paraffin embedded rat lung sections using Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (#ASR-011), (1:100). Staining is present in vascular smooth muscle (black arrows) but not in the muscular layer of bronchi (red arrows). Hematoxilin is used as the counterstain. |
| Immunocytochemistry: |
| Expression of Sphingosine 1-Phosphate Receptor 1 in mouse 3T3 cells |
 |
| Immunocytochemical staining of mouse 3T3 cells using Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody (#ASR-011), (1:50), (red). DAPI was used for nuclear staining (blue). |
Lysophospholipids constitute a group of important lipid mediators; lysophosphatidic acid (LPA) a glycerolysophospholipid and sphingosine 1-phosphate (SIP), a lysosphingolipid. Sphingosine 1-phosphate is a bioactive lipid derived from metabolism of sphingomyelin1. Sphingosine 1-phosphate is implicated in the regulation of many cellular functions including proliferation, apoptosis, survival, adhesion, differentiation, and migration2. Sphingosine 1-phosphate exerts its activity through five distinct G-protein-coupled receptors, (also named endothelial differentiation gene receptors - EDG); S1PR1 (EDG-1), S1PR2 (EDG-5), S1PR3 (EDG-3), S1PR4 (EDG-6), and S1PR5 (EDG-8)3.
Sphingosine 1-Phosphate Receptor 1 (S1PR1) is ubiquitously expressed. S1PR1 mRNA is detected in the brain, heart, spleen, liver, lung, thymus, kidney, skeletal muscle and lymphoid tissues4.
Knockout of the gene encoding S1PR1 is lethal and therefore makes in vivo studies difficult 5.
Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the human Sphingosine 1-Phosphate Receptor 1. Anti-Sphingosine 1-Phosphate Receptor 1 (extracellular) antibody, (#ASR-011) can be used for western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize Sphingosine 1-Phosphate Receptor 1 from human, rat and mouse samples.