Anti-SP4 Antibody

Anti-SP4 Antibody LS-B3521

SP4 Rabbit anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3521 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3521 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. ELISA titer in peptide based assay: 1:1562500. Learn more

SP4 Antibody Details

 

 

Target:

Human SP4

 

Host:

Rabbit

 

Reactivity:

Human, Mouse, Rat

 

Clonality:

IgG Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml)

 

Western blot (WB) (0.2 - 1 µg/ml)

 

Optimal dilution to be determined by the researcher.

 

SP4 Antibody Specificity

 

Immunogen:

SP4 antibody was raised against synthetic peptide from internal region of human SP4 (NP_003103.2, Q02446). Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Pig, Xenopus (100%); Guinea pig (92%).

 

Epitope:

Internal

 

Specificity:

Human SP4

SP4 Antibody Packaging

 

Presentation:

Lyophilized from PBS with 2% sucrose

 

Storage:

Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

 

Restrictions:

For research use only.