Anti-SOX4 Antibody

SOX4 Rabbit anti-Human Polyclonal (N-Terminus) Antibody



An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3520 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3520 was determined to be 5 ug/ml. ELISA titer using peptide based assay: 1:1562500. Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody. Learn more

SOX4 Antibody Details
 
 


Target:
Human SOX4
 
Host:
Rabbit
 
Reactivity:
Human, Mouse, Rat
 
Clonality:
IgG Polyclonal
 
Purification:
Immunoaffinity Chromatography
 

Applications:
 
Immunohistochemistry - Paraffin (IHC-P) (5 µg/ml)
 
Western blot (WB)
 
Optimal dilution to be determined by the researcher.
 
SOX4 Antibody Specificity
 


Immunogen:
SOX4 antibody was raised against synthetic peptide from N-terminus of human SOX4 (NP_003098.1, Q06945). Percent identity by BLAST analysis: Human, Mouse, Dog, Bovine, Rabbit, Guinea pig, Chicken, Xenopus, Zebrafish (100%).
 
Epitope:
N-Terminus
 
Specificity:
Human SOX4


SOX4 Antibody Packaging
 


Presentation:
Lyophilized from PBS with 2% sucrose
 
Storage:
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
 
Restrictions: