Anti-Somatostatin Receptor Type 4 (extracellular)
- 貨號 Cat. No.
- ASR-004
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Somatostatin Receptor Type 4 (extracellular)
| Product#: | ASR-004 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit. |
| Epitope: |
Peptide DTRPARGGEAVAC corresponding to residues 182-194 of rat SSTR4 (Accession # P30937). |
 |
| Putative epitope location: |
| 2nd extracellular loop. |
| Homology: |
Mouse- identical, human ?12/13 residues identical. |
| Reactivity Confirmed: |
| Rat. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain lysate: 1. Anti-SSTR4 (extracellular) antibody, (#ASR-004), (1:200). 2. Anti-SSTR4 (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of SSTR4 in rat hippocampus |
 |
Immunohistochemical staining of somatostatin receptor 4 (SSTR4) with anti-SSTR4 (extracellular) antibody in rat hippocampus. A, SSTR4 appears in the pyramidal layer (red). B, Staining of interneurons with mouse anti parvalbumin (PV, green). C, Confocal merge of SSTR4 and PV demonstrates separate localization in hippocampus. |
| References: |
| 1. Patel, Y.C. (1999) Front Neuroendocrin. 20,157. |
| 2. Weckbecker, G. et al. (2003) Nat. Rev. Drug Discov. 2, 999. |
| 3. Culler, M.D. et al. (2002) Ann. Endocrinol. (Paris) 63, 2S5. |
| 4. Corleto, V.D. et al. (2004) Dig. Liver Dis. 36, S8. |
Somatostatin (SST) is a small cyclic peptide that was first identified as a powerful inhibitor of the secretion of various hormones including growth hormone (GH), thyroid-stimulating hormone (TSH) and prolactin from the pituitary, as well as practically every major hormone from the intestinal tract. SST consists of two major bioactive forms, SST-14 and the N-terminus extended peptide SST-28 that can be produced by a wide variety of neuroendocrine, inflammatory and immune cells. In target cells SST induces a variety of physiological functions that include neuromodulation, cell secretion, cell proliferation and smooth muscle contractility.
SST acts on its multiple cell targets via a family of six receptors that originate from five genes: SSTR1, SSTR2a, SSTR2b, SSTRR3, SSTR4, SSTR5. The SSTRs are members of the G-protein coupled receptor superfamily and they modulate cell response via multiple second messenger systems such as inhibition of adenylate cyclase, modulation of conductance of ion channels and protein dephosphorylation.
SSTRs are expressed widely in both the brain and peripheral tissues but with receptor subtype variations between the different cell types. Thus, SSTR4 is the least SSTR subtype expressed in the brain but is most strongly expressed in the lung, heart and placenta.
Strong SSTR expression has been detected in a variety of human tumors including pancreatic, breast, lung and prostate cancers. In fact, SST has been shown to inhibit the growth of various normal and tumor cells. On this basis, several long lasting SST analogs have been developed and are being tested for use in several pathologies such as various cancers, acromegaly, immunoproliferative disorders, diabetic retinopathy, epilepsy and pain.
We have now developed a new antibody against a highly conserved epitope in the second extracellular loop of the rat SSTR4. The antibody will also recognize mouse and human samples.