Anti-Somatostatin Receptor Type 2 (extracellular)
- 貨號 Cat. No.
- ASR-006
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Somatostatin Receptor Type 2 (extracellular)
| Product#: | ASR-006 |
| Sizes: |
50µl |
0.2ml |
| Host: |
Rabbit. |
| Epitope: |
| Peptide (C)GSNQTEPYYDMTSN corresponding to residues 30-43 of rat SSTR2 (Accession number P30680). |
| Putative epitope location: |
Extracellular, N-terminus. |
 |
| Homology: |
| Mouse - identical; human, pig - 12/15 residues identical. |
| Reactivity Confirmed: |
| Rat and mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of rat brain (lanes 1,3) and rat pancreas (lanes 2 and 4) lysates: 1. Anti-SSTR2 (extracellular) antibody (#ASR-006) (1:200). 2. Anti-SSTR2 (extracellular) antibody preincubated with the control peptide antigen. |
| Immunohistochemistry: |
Expression of SSTR2 in rat hippocampus and mouse cortex |
 |
Immunohistochemical staining of somatostatin receptor 2 (SSTR2) with Anti-SSTR2 (extracellular) antibody (# ASR-006) in rat hippocampus. (A) SSTR2 appears in neural processes (green) that are perpendicular to dentate granule layer (arrow). (B) Staining of axons with mouse anti Neurofilament 200 (NF200, red). (C) Confocal merge of SSTR2 and NF200 images suggests that SSTR2 is not present in axons that run through the granule layer and hilus, but rather in neuronal dendrites that ascend toward the dentate hilus (arrow). |
 |
| Immunohistochemical staining of somatostatin receptor 2 (SSTR2) in mouse cortex. Frozen section of mouse cortex was visualized using Anti-SSTR2 (extracellular) antibody (#ASR-006) (1:100). (A) SSTR2 appears in neural processes (green, horizontal arrow) that are perpendicular to the cortical surface (vertical arrow). (B) Staining of axons with mouse anti-Neurofilament 200 (NF200, red) appears mostly in the deep layers. (C) Confocal merge of SSTR2 and NF200 images suggests that SSTR2 is not present in axons that run in the deep layers, but rather in neuronal dendrites that ascend toward upper layers of cortex (vertical arrow). |
| References: |
| 1. Hofland, L.J. and Lamberts, S.W.J. (2001) Ann. Oncol. 12, S31. |
| 2. Fombonne, J. et al. (2003) Rep. Biol. Endocrinol. 1, 19. |
| 3. Slooter, G.D. et al. (2001) Br. J. Surg. 88, 31. |
| 4. Schulz, S. et al. (2002) Gyn. Oncol. 84, 235. |
Somatostatin is a small cyclic peptide that is widely expressed throughout the central nervous system and peripheral tissues.1 In peripheral tissues, somatostatin exerts inhibitory effects on secretion processes, whereas in the brain, it acts as a neurotransmitter in both a stimulatory and an inhibitory manner.1,2
Somatostatin mediates its action via six high affinity G-protein-coupled receptors (SSTR1, SSTR2a, SSTR2b, SSTR3, SSTR4, and SSTR5), which are encoded by five genes.1,2 Expression of the different receptors is developmentally regulated in a time- and tissue-specific manner.2
Somatostatin receptors have been found on a variety of neuroendocrine tumors, such as paragangliomas, carcinoids, and breast tumors.3 Synthetic peptide derivatives of somatostatin have been successfully used in the treatment of neuroendocrine malignancies and in vivo imaging of tumors that are positive to somatostatin receptors.4
In general, SSTR2 is the most common SSTR subtype found in human tumors, followed by SSTR1, with SSTR3 and SSTR4 being less common.