Anti-SOCS3 Antibody

Anti-SOCS3 Antibody LS-B3317

SOCS3 Mouse anti-Human Monoclonal (SO1) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3317 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3317 was determined to be 10 ug/ml. Western Blot: LS-B3317 detects a band of approximately 30kD in Hela cell lysates. Learn more

SOCS3 Antibody Details

 

 

Target:

Human SOCS3

 

Host:

Mouse

 

Reactivity:

Human

 

Clonality:

IgG2b Monoclonal [SO1]

 

Purification:

Protein A Column

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 μg/ml)

 

Western blot (WB)

 

ELISA (1:100 - 1:500)

 

Optimal dilution to be determined by the researcher.

 

SOCS3 Antibody Specificity

 

Immunogen:

SOCS3 antibody was raised against full - length SOCS3 protein.

 

Specificity:

Recognizes suppressor of cytokine signaling 3 (SOCS3), a 30kD member of the suppressor of cytokine signaling family (SOCS) which negatively regulate Janus Kinase (JAK) and signal transducer and activators of transcription (STAT) signaling pathways. SOCS3 specifically acts as a negative feedback regulator for the STAT3 pathways, by directly binding to the Tyr-759 motif of the IL-6 signal-transducing receptor subunit gp130, thereby preventing the IL-6 activation of STAT3. The expression of SOCS3 is inducible by numerous factors, including IL-6, IL-10, Toll-like receptor (TLR) agonists and IFN gamma, making this protein the focus of research into inflammatory diseases, such as Arthritis, Colitis, and Crohns Disease. SOCS3 expression has also been shown to affect IFN alpha sensitivity in tumor studies.

SOCS3 Antibody Packaging

 

Presentation:

PBS, pH 7.4, 0.09% sodium azide.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.