Anti-SNAP25 Antibody

Anti-SNAP25 Antibody LS-B3684

SNAP25 Mouse anti-Human Monoclonal (SP12) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3684 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D2, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3684 was determined to be 0.2-0.5 ug/ml. Learn more

SNAP25 Antibody Details

 

 

Target:

Human SNAP25

 

Host:

Mouse

 

Reactivity:

Hamster, Human, Porcine, Rat

 

Clonality:

IgG1 Monoclonal [SP12]

 

Purification:

Supernatant

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (0.2 - 0.5)

 

Western blot (WB) (1:500 - 1:5000)

 

ELISA

 

Optimal dilution to be determined by the researcher.

 

SNAP25 Antibody Specificity

 

Immunogen:

SNAP25 antibody was raised against crude human synaptic immunoprecipitate.

 

Specificity:

Appears to recognize the human pre-synaptic protein, SNAP-25; an antigen of 26-27kD molecular weight. It will recognize SNAP-25 fusion protein from COS cells, but not with the fusion protein from bacterial systems. Research studies have used this antibody to study the distribution of synaptic changes in the hippocampus of patients with medically refractory temporal lobe epilepsy.

SNAP25 Antibody Packaging

 

Presentation:

PBS, pH 7.2, 0.09% sodium azide.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.