Anti-SEPT11 / Septin 11 Antibody

Anti-SEPT11 / Septin 11 Antibody LS-B4024

SEPT11 / Septin 11 Rabbit anti-Human Polyclonal (N-Terminus) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B4024 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4024 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. Learn more

SEPT11 / Septin 11 Antibody Details

Target: Human SEPT11 / Septin 11

Host: Rabbit

Reactivity: Human, Mouse, Rat

Clonality: IgG Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (5)

Western blot (WB) (0.2 - 1)

Optimal dilution to be determined by the researcher.

 

SEPT11 / Septin 11 Antibody Specificity

Immunogen: SEPT11 / Septin 11 antibody was raised against synthetic peptide from N-terminus of human SEPT11(septin 11) (NP_060713.1, Q9NVA2). Percent identity by BLAST analysis: Human, Orangutan, Monkey, Mouse, Rat, Bovine (100%).

Epitope: N-Terminus

Specificity: Human SEPT11 / Septin 11

SEPT11 / Septin 11 Antibody Packaging

Presentation: Lyophilized from PBS with 2% sucrose

Storage: Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.

Restrictions: For research use only.