Anti-SARS-CoV-2?Nucleocapsid?(NP)?Mouse?IgG?ELISA?Kit
- 規格 Size
- 96 T
- 品牌 Brand
- AntibodySystem
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Nucleocapsid (NP) protein has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Nucleocapsid (NP) Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP-labeled antibody specific for Mouse IgG is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Mouse SARS-CoV-2 Nucleocapsid (NP) IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 Nucleocapsid (NP) protein has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 Nucleocapsid (NP) Mouse IgG present is bound by the immobilized protein. After washing away any unbound substances, a HRP-labeled antibody specific for Mouse IgG is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Mouse SARS-CoV-2 Nucleocapsid (NP) IgG bound in the initial step. The color development is stopped and the intensity of the color is measured.
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