Anti-SARS-CoV-2 RBD (BA.5) Human IgA ELISA Kit
- 規格 Size
- 96 T
- 品牌 Brand
- AntibodySystem
- Application 應用
- Used for the quantitative determination of Anti-SARS-CoV-2 RBD (BA.5) Human IgA concentration in serum and plasma.
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 RBD (BA.5) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 RBD (BA.5) Human IgA present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgA antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 RBD (BA.5) Human IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.
This assay employs the quantitative indirect enzyme immunoassay technique. Recombinant SARS-CoV-2 RBD (BA.5) has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Anti-SARS-CoV-2 RBD (BA.5) Human IgA present is bound by the immobilized protein. After washing away any unbound substances, a biotin-labeled Mouse Anti-Human IgA antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-SARS-CoV-2 RBD (BA.5) Human IgA bound in the initial step. The color development is stopped and the intensity of the color is measured.
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