Anti-S100A9 Antibody

Anti-S100A9 Antibody LS-B3613

S100A9 Goat anti-Human Polyclonal (Internal) Antibody

An IHC-plusTM AntibodyImmunohistochemistry: LS-B3613 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3613 was determined to be 2.5 ug/ml. Peptide ELISA: antibody detection limit dilution 1:8000. Western Blot: Approx 16kD band observed in Human Peripheral Mononucleocytes lysates (calculated MW of 13.2kD according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kD) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16;280(50):41521-9.; PMID: 16216873). Recommended concentration: 0.3-1 ug/ml. Learn more

S100A9 Antibody Details

Target: Human S100A9

Host: Goat

Reactivity: Human

Clonality: Polyclonal 

Purification: Immunoaffinity Chromatography

 Applications:

Immunohistochemistry - Paraffin (IHC-P) (2.5)

Western blot (WB) (0.3 - 1)

ELISA (1:8000) & (0.3 - 1)

Optimal dilution to be determined by the researcher.

 

S100A9 Antibody Specificity

Immunogen: S100A9 antibody was raised against synthetic peptide C-DTNADKQLSFEEF from an internal region of human S100A9 (NP_002956.1). Percent identity with other species by BLAST analysis: Human (100%), Gorilla (100%), Gibbon (100%), Monkey (100%), Marmoset (92%), Hamster (92%), Bovine (92%), Rabbit (92%), Horse (92%), Pig (92%), Panda (85%).

Epitope: Internal

Specificity: Human S100A9.

S100A9 Antibody Packaging

Presentation: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide

Storage: Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

Restrictions: For research use only.