Anti-Ryanodine Receptor 2

Anti-Ryanodine Receptor 2

Western blot analysis of rat heart membrane: 1.       Anti-Ryanodine receptor 2 antibody (#ARR-002), (1:200). 2.       Anti-Ryanodine receptor 2 antibody, preincubated with the control peptide antigen.

Immunohistochemical staining of paraffin-embedded section of rat myocardium using Anti-RyR2 antibody (#ARR-002), (1:50). Staining is specific for cardiomyocytes while smooth muscles cells in the artery walls are negative (red arrows). Hematoxylin is used as the counterstain.         Expression of Ryanodine Receptor 2 (RyR2) in mouse cerebellum

Immunohistochemical staining of Ryanodine receptor RyR2 in mouse cerebellum frozen section using  Anti-RyR2 antibody (#ARR-002) (1:100) (green fluorescence).   A, the highest expression of RyR2 is in the molecular layer (Asterik) but there is also some expression in the soma of Purkinje cells (arrows). B, In the same section, there is staining for parvalbumin (red), a marker of Purkinje cells. C, merging the two images demonstrates that RyR2 is localized both surrounding the dendritic tree and in the soma of Purkinje cells. Blue is DAPI counterstain

It is well established that cytosolic calcium (Ca²⁺) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.^1,2  The primary intracellular Ca²⁺ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

The ER and SR contain two Ca²⁺ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).³

The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).³ The Ryanodine receptors are homotetrameric proteins. They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.

RyR2 serves as an intracellular Ca²⁺ channel in the SR membrane. It is predominantly expressed in cardiac muscle where it plays a central role in cardiac excitation-contraction coupling. RyR2 is also expressed in the brain. ^1-4

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the cytoplasmic region of the human Ryanodine receptor 2 (RyR2). The Anti-RyR2 antibody (#ARR-002) can be used for Western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR2 from human, rat and mouse samples.