Anti-Ryanodine Receptor 1

 

Product#:

ARR-001

Sizes:

50 µl

0.2 ml

Host:

Rabbit.     Type: Polyclonal.

Epitope:

Peptide (C)RAENEKDATTEKNKKR corresponding to amino acid residues 1371-1386 of human Ryanodine receptor 1(Accession  P21817).

Putative epitope location:

Intracellular, N-terminus.

Homology:

Rat, mouse ?identical.

Reactivity Confirmed:

Rat, mouse.

Applications:

Western Blotting:

Western blot analysis of rat skeletal muscle lysate: 1.       Anti-Ryanodine receptor 1 antibody (#ARR-001), (1:200). 2.       Anti- Ryanodine receptor 1 antibody, preincubated with the control peptide antigen.

Immunohistochemistry:

Expression of Ryanodine Receptor 1 (RyR1) in rat skeletal muscle

Immunohistochemical staining of paraffin embedded section of rat quadriceps using Anti-RyR1 antibody (#ARR-001) (1:100). RyR1 is expressed in fibers of striate muscle. Note that smooth muscle in the arterial walls is negative as well as the surrounding connective tissue.  Counterstain is Hematoxilin.       Expression of Ryanodine Receptor 1 (RyR1) in rat cerebellum

Immunohistochemical staining of Ryanodine receptor 1 (RyR1) in rat cerebellum frozen section using Anti-RyR1 antibody (#ARR-001) (1:100) (green fluorescence). A, the highest expression of RyR1 is in the molecular layer (Asterik). B, In the same section, there is staining for parvalbumin (red), a marker of Purkinje cells. C, merging the two images demonstrates that RyR1 is localized surrounding the dendritic tree of Purkinje cells. Blue is DAPI counterstain

It is well established that cytosolic calcium (Ca²⁺) acts as a key second messenger in many intracellular pathways including synaptic transmission, muscle contraction, hormonal secretion, cell growth and proliferation.^1,2  The primary intracellular Ca²⁺ storage/release organelle in most cells is the endoplasmic reticulum (ER) or the sarcoplasmic reticulum (SR) in striated muscle cells.

The ER and SR contain two Ca²⁺ release channels families, the Inositol trisphosphate receptors (IP3Rs) and the Ryanodine receptors (RyRs).³

The Ryanodine receptor family consists of three different isoforms: The skeletal muscle isoform, Ryanodine Receptor type 1 (RyR1); the cardiac muscle isoform, Ryanodine Receptor type 2 (RyR2) and the brain isoform, Ryanodine Receptor type 3 (RyR3).³ The Ryanodine receptors are homotetrameric proteins generating a Ca²⁺ conducting channel . They play a key role in the mechanism of excitation-contraction coupling in striated muscle. Binding of Ryanodine to the Ryanodine Receptor causes to two major changes in the channel: a reduction in single-channel conductance and a marked increase in open state probability.

 

The RyR1 is expressed predominantly in skeletal muscles and areas of the brain; the human RyR1 has at least three known alternative spliced variants.⁵

 

Several diseases are attributed to mutations in RyR1 gene: Central Core Disease (CCD), multi-minicore disease (MmD) and Malignant Hyperthermia (MH).⁵

 

Alomone Labs is pleased to offer a highly specific antibody directed against an an epitope located at the cytoplasmic region of the human Ryanodine receptor 1 (RyR1). The Anti-RyR1 antibody (#ARR-001) can be used for Western blot analysis and Immunohistochemical applications. It has been designed to recognize RyR1 from human, rat and mouse samples.