Anti-Rituximab ELISA Kit
- 規格 Size
- 96 T
- 品牌 Brand
- AntibodySystem
- Application 應用
- Used for the quantitative determination of Anti-Rituximab concentration in serum and plasma.
Description
PRINCIPLE OF THE ASSAY
This assay employs the quantitative sandwich enzyme immunoassay technique. Rituximab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Rituximab will be captured by immobilized Rituximab. After washing away any unbound substances, a biotin-labeled Rituximab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Rituximab bound in the initial step. The color development is stopped and the intensity of the color is measured.
This assay employs the quantitative sandwich enzyme immunoassay technique. Rituximab has been pre-coated onto a microplate. Samples or standards are pipetted into microwells and Anti-Rituximab will be captured by immobilized Rituximab. After washing away any unbound substances, a biotin-labeled Rituximab is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Anti-Rituximab bound in the initial step. The color development is stopped and the intensity of the color is measured.
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