Anti-rat p75 neurotrophin receptor

Anti-rat p75 neurotrophin receptor

Product#:

AN-170

Sizes:

10 μg

50 μg

p75^NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor  

for b-amyloid, and for the myelin axon repellant protein MAG.^1,2,4,6 

Binding of neurotrophins induces receptor dimerization followed by phosphorylation of  

receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. 

p75^NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB  

and trkC. p75^NTR signals via interaction with different transducers such as TRAF6, IRAK,  

and RIP-2, activating NF-kappaB, JNK and p38, and inactivating rhoA.^1,3,6 



Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain. 

p75^NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity,  

and is more potently activated by pro-neurotrophins.⁵  



Overexpression of the intracellular domain in developing neurons induces apoptosis.  

NGF-dependent association of NRIF with p75^NTR contributes to pro-apoptotic signaling.^7,8 

Beta-amyloid peptide binds p75^NTR and stimulates pro-apoptotic signaling by p75^NTR,  

suggesting that the receptor may be relevant for Alzheimer's disease.⁹

Description:

Mouse monoclonal.

Clone:

192-IgG

Isotype:

IgG1.

Immunogen:

Solubilized proteins from PC12 membranes.

Specificity:

192-IgG reacts with rat p75. It does not cross-react with p75 of human or mouse origin.

Purity:

Purified from cultured hybridoma medium on immobilized rProtein G.

Applications:

Immunohistochemistry:

Immunohistochemical staining of the rat diagnonal band nucleus, demonstrating partial co-localization of the p75 receptor using Anti-p75 (#AN-170) and nitric oxide synthase (red fluorescence, Anti-NOS, Sigma).

Western Blotting:

Western blotting of PC12 cell lysate (non-reduced).

Anti-rat p75 (1:2500). Anti-rat p75 (1:1500). Anti-ratp75 (1:150).

References:

1.	Dechant, G. et al. (1997) Curr. Opin. Neurobiol. 7, 413.
2.	Cosgaya, J.M. et al. (2002) J. Science 298, 1245.
3.	Yamashita, T. et al. (2003) Nature Neurosci. 6, 461.
4.	Bentley, C.A. et al. (2000) J. Neurosci. 20, 7706.
5.	Lee, R. et al. (2001) Science 294, 1945.
6.	Bibel, M. et al. (2000) Genes Dev. 14, 2919.
7.	Salehi, A.H. et al. (2002) J. Biol. Chem. 277, 48043.
8.	Casademunt, E. et al. (1999) EMBO J. 18, 6050.
9.	Yaar, M. et al. (1997) J. Clin. Invest. 100, 2333.