Anti-Rat H2 Histamine Receptor (extracellular)
- 貨號 Cat. No.
- AHR-002
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Rat H2 Histamine Receptor (extracellular)
| Product#: | AHR-002 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
Peptide RNGTRGGNDTFKC, corresponding to amino acids 161-173 of rat H2 Histamine Receptor (Accession P25102). |
 |
| Putative epitope location: |
Extracellular, 2nd loop. |
| Homology: |
Mouse- identical. Wonî–¹ recognize human H2 Histamine Receptor. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat basophilic leukemia cells (RBL) (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): 1, 2. Anti- Rat H2 Histamine Receptor (extracellular) antibody (#AHR-002) (1:200). 3, 4. Anti- Rat H2 Histamine Receptor (extracellular), preincubated with the control peptide antigen |
| Immunohistochemistry: |
 |
Expression of H2 Histamine Receptor in rat stomach. Immunohistochemical staining of paraffin embedded section of rat stomach using Anti-Rat H2 Histamine Receptor (extracellular) antibody (#AHR-002) (1:100). H2R (brown staining) is expressed in parietal cells (arrows) of the gastric glands. Counterstain is Hematoxilin. |
| Flow Cytometry: |
 |
Flow cytometer analysis of H2 Histamine Receptor expression in live intact RBL (rat basophilic leukemia) cell lines.
Black line: Control cells + Goat-anti-Rabbit-FITC
Green line: Cells+ Anti-Rat H2 Histamine Receptor (extracellular) antibody (#AHR-002) (1:20) + Goat-anti-Rabbit-FITC |
Histamine (2-[4-imidazole]ethylamine) is a low-molecular-weight amine synthesized from L-histidine. It is produced by various cells throughout the body, including central nervous system neurons, gastric mucosa parietal cells, mast cells, basophils and lymphocytes. Histamine is a major biological mediator whose functions include, among many others, regulation of vascular smooth muscle, immune regulation, regulation of sleep-wake cycles and regulation of gastric acid secretion.1,2
The biological effects of histamine are mediated through four receptors (H1- H4 Histamine receptors) all of which belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.
H2 receptors couple to adenylate cyclase via Gs, leading to the formation of cAMP and the activation of protein kinase A (PKA).1,2
H2 receptors are expressed in several tissues including brain, gastrointestinal tract and heart.
The most studied physiological role of the H2 receptors is their involvement in the regulation of gastric acid secretion.3 Specific H2 receptor antagonists are currently used for the treatment of acid-related gastrointestinal conditions such as peptic ulcer, gastroesophageal reflux disease and dyspepsia.
Involvement of H2 receptors in chronic heart failure and immune regulation has also been suggested.
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the 2nd extracellular loop of the rat H2 Histamine receptor. Anti-Rat H2 Histamine receptor (extracellular) antibody (#AHR-002) can be used in Western blot, indirect flow cytometry and immunohistochemical applications, and recognizes H2 Histamine receptor from rat and mouse samples.