Anti-RAB3A Antibody

Anti-RAB3A Antibody LS-B3406

RAB3A Rabbit anti-Human Polyclonal (aa202-217) Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3406 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D1, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3406 was determined to be 10 ug/ml. Learn more

RAB3A Antibody Details

 

 

Target:

Human RAB3A

 

Host:

Rabbit

 

Reactivity:

Human, Mouse, Rat

 

Clonality:

 Polyclonal

 

Purification:

Immunoaffinity Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (10 µg/ml)

 

Western blot (WB)

 

Optimal dilution to be determined by the researcher.

 

RAB3A Antibody Specificity

 

Immunogen:

RAB3A antibody was raised against a 16 residue synthetic peptide (C)KQGPQLSDQQVPPHQD based on the human Rab3A (residues 202-217) with the cysteine (C) residue added and the peptide coupled to KLH.

 

Epitope:

aa202-217

 

Specificity:

Detects an ~25 kD protein, corresponding to the apparent molecular mass of Rab3A on SDSPAGE immunoblots, in samples from mouse and rat origins. The antibody specificity is confirmed in peptide inhibition immunoblotting studies.

RAB3A Antibody Packaging

 

Presentation:

Borate buffered saline, pH 8.2 (25mM sodium borate, 100mM boric acid, 75mM NaCl, 5mM EDTA (disodium salt)

 

Storage:

Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.

 

Restrictions:

For research use only.