Anti-Pyruvate Kinase Antibody

Anti-Pyruvate Kinase Antibody LS-B3658

Pyruvate Kinase Goat anti-Rabbit Polyclonal Antibody

An IHC-plusTM Antibody

 Immunohistochemistry: LS-B3658 was validated for use in immunohistochemistry on a panel of formalin-fixed, paraffin-embedded (FFPE) rabbit tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody LS-D3, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3658 was determined to be 5 ug/ml. Suitable for use in Flow Cytometry, ELISA, Western Blot (Western and Dot Blot), Immunoprecipitation, Conjugation. Learn more

Pyruvate Kinase Antibody Details

 

 

Target:

Rabbit Pyruvate Kinase

 

Host:

Goat

 

Reactivity:

Human, Rabbit

 

Clonality:

IgG Polyclonal

 

Purification:

Ion Exchange Chromatography

 

Applications:

 

Immunohistochemistry - Paraffin (IHC-P) (5)

 

Western blot (WB)

 

Immunoprecipitation (IP)

 

Flow Cytometry (Flo)

 

ELISA (1:35000 - 1:150000)

 

Optimal dilution to be determined by the researcher.

 

Pyruvate Kinase Antibody Specificity

 

Immunogen:

Pyruvate Kinase antibody was raised against pyruvate Kinase from rabbit muscle.

 

Specificity:

Recognizes all forms of Pyruvate Kinase. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum as well as purified and partially purified Pyruvate Kinase (Rabbit Muscle). Species cross-reactivity: Expected to cross-react Pyruvate Kinase from other mammals.

Pyruvate Kinase Antibody Packaging

 

Presentation:

PBS, pH 7.2, 0.1% sodium azide, 40% glycerol. No stabilizing proteins added.

 

Storage:

+4°C or -20°C, Avoid repeated freezing and thawing.

 

Restrictions:

For research use only.