Anti-proNT-3
- 貨號 Cat. No.
- ANT-012
- 規格 Size
- 50 μl 0.2ml
Description
Anti-proNT-3
| Product#: | ANT-012 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
Peptide (C)DTELLRQQRRYNSPR, corresponding to amino acid residues 89-103 of human NT-3 (precursor) (Accession P20783). |
 |
| Putative epitope location: |
Pro domain of the NT-3 protein. |
| Homology: |
Rat, mouse ?identical. Specificity: The antibody is specific for proNT-3, it does not crossreact with proNGF, proBDNF or mature NT-3. |
| Reactivity Confirmed: |
| Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting using Anti-proNT-3 antibody (1:600): 1. hNT-3 200 ng. 2. hNT-4 200 ng. 3. mNGF 200 ng. 4. hNGF 200 ng. 5. hBDNF 200 ng. 6. recombinant proNGF 200 ng. 7. recombinant proBDNF 200 ng. 8. recombinant proNT-3 10 ng. |
 | Western blotting of mouse recombinant proNT-3: 1, 5. 10 ng proNT-3. 2, 6. 50 ng proNT-3. 3, 7. 100 ng proNT-3. 4, 8. 200 ng proNT-3. Lanes 1-4 Anti-proNT-3 antibody (1:300). Lanes 5-8 Anti-proNT-3 antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
| Immunohistochemical staining of proNT-3 in the rat Islands of Calleja (IOC) using Anti-proNT-3 antibody (#ANT-012). A representative island is shown in A: proNT-3 staining (green) overlaps neuronal nitric oxide synthase (nNOS red) while a cluster of cells covers a part of the island (DAPI blue counterstain) but not a cell-poor area (white star). In B, the distribution of proNT-3 is shown alone. Note that staining is more intense in the cell poor area demarcated by white line. In C, the distribution of neuronal NOS is most intense in the cell-poor region. |
| References: |
| 1. Hempstead, B. (2002) Curr. Opin. Neurobiol. 12, 260. |
| 2. Blum, R. and Konnerth, A. (2005) Physiology 20, 70. |
Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF) neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).
All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.
Alomone Labs has now developed a new antibody directed against a highly specific epitope located in the pro domain region of human NT-3. The antibody does not cross reacts with mature NT-3, pro and mature NGF or pro and mature BDNF.