Anti-proNGF

Anti-proNGF

Product#:

ANT-005

Sizes:

50 μl

0.2 ml

Host:

Rabbit.

Description:

Polyclonal antibody raised in rabbit, affinity purified on immobilized antigen.

Epitope:

Peptide (C)SPRVLFSTQPPPTSSDTLDLD, corresponding to amino acid residues 84-104 of rat NGF (precursor) (Accession number P25427).

Putative epitope location:

Pro domain of the NGF protein.

Homology:

Mouse-identical, human-16 out of 16/21 residues identical.

Reactivity Confirmed:

His-fusion protein containing the entire prodomain of rat proNGF.

Specificity:

The antibody is specific for proNGF, it does not crossreact with proBDNF, proNT-3 or mature NGF.

Applications:

Immunohistochemistry:

Rat brain sections.

ProNGF localization in glial cells of the rat corpus callosum.  Staining of astrocytes was performed using mouse anti-glial fibrillary acidic protein (GFAP), labeled red (A).  Anti-proNGF (#ANT-005) was labeled green (C). Co-localization is demonstrated in (B).

Western Blotting:

Western blotting with anti-proNGF antibody: 1.  His-prodomain fusion protein, Anti-proNGF antibody (1:500). 2.  His-prodomain fusion protein, Anti-proNGF antibody (1:500) preincubated with the control peptide antigen

Western blotting of mice submanibular glands (SMGs): 1.  Anti-proNGF antibody (1:200). 2.  Anti-proNGF antibody (1:200), preincubated with the control peptide antigen.

Control antigen included in price

References:

1.	Lessman V. et al. (2003) Progress in Neurobiology 69, 341.
2.	Bibel, M. and Barde, Y.A. et al. (2000) Genes and Development 14, 2929.
3.	Lee, R et al. (2001) Science 294, 1945.
4.	Ibanez, C. F.et al. (2002) Trends in Neurosci. 25, 284.
5.	Hassan, W. et al. (2003) J. Neurobiol. 57, 38.
6.	Beattie, M. S. et al. (2002) J. Neuron. 36, 375.
7.	Fahnestock, M. et al. (2001) J. Mol Cell Neurosci. 18, 210.
8.	Lu, B. et al. (2003) Neuron. 39, 735.

Neurotrophins are synthesized as pro-forms that can be cleaved either intracellularly to  

release mature, secreted ligands, or extracellularly by various proteases such as plasmin,  

furin, PC1/3, PC7, and PACE 4.^1,2,5 The immature precursor has a prodomain of 103  

amino acids, which was thought to have a role in the folding and sorting of the mature  

NGF into the various secretion pathways. 



It was recently reported that proNGF, binds p75^NTR receptor preferentially over TrkA,  

and this selective binding of proNGF to p75^NTR  leads to apoptotic death of cells that  

express both TrkA and p75^NTR. However, mature NGF binds and activates both  

receptors, with resulting promotion of cell survival due to the TrkA-mediated survival  

signal overriding p75^NTR -mediated apoptotic signal.^3,4 



Since pro- and mature neurotrophins seem to elicit opposite functional effects, by  

differential interactions with Trks and p75^NTR receptors, extracellular cleavage  

represents a new way to control the synaptic functions of neurotrophins.  

It was demonstrated that proNGF from injured spinal cord extracts, is active and  

induce apoptosis among oligodendrocytes, and apoptosis can be blocked by  

a proNGF-specific antibody.⁶  



Finally, proNGF was demonstrated as the predominant form in mouse, rat, and  

human brain tissue, thyroid, hippocampus, thus suggesting a role for proNGF in vivo.^5,7,8