Anti-Prokineticin Receptor-1 (extracellular)
- 貨號 Cat. No.
- APR-041
- 規格 Size
- 50 μl 0.2ml
Description
Anti-Prokineticin Receptor-1 (extracellular)
| Product#: | APR-041 |
| Sizes: |
| 50 µl |
| 0.2 ml |
| Host: | ||
Rabbit.
|
| Epitope: |
| Peptide(C)ENTTNTFTDFFSARD corresponding to amino acid residues 10-24 of rat Prokineticin receptor 1 (Accession Q8R416). |
 |
| Putative epitope location: |
Extracellular, N-terminus. |
| Homology: |
Mouse -12 out of 15 residues identical. Unlikely to recognize human samples. |
| Reactivity Confirmed: |
Rat, mouse. |
Applications:
| Western Blotting: |
 | Western blot analysis of mouse brain lysate: 1. Anti-Prokineticin Receptor 1 (extracellular) antibody (#APR-041), 1:200. 2. Anti-Prokineticin Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen. |
 | Western blot analysis of rat brain membranes: 1. Anti-Prokineticin Receptor 1 (extracellular) antibody (#APR-041), 1:200. 2. Anti-Prokineticin Receptor 1 (extracellular) antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
Immunohistochemical staining of frozen sections of rat dorsal root ganglion (DRG). Sections were stained with Anti-Prokineticin Receptor 1 (extracellular) antibody (#APR-041), (1:100) followed by Alexa-555-conjugated goat anti-rabbit secondary antibody. PKR1 (red staining) is expressed in DRG neurons. Cell nuclei are visualized with Hoechst 33342 (blue staining). |
 |
Immunohistochemical staining of mouse olfactory bulb brain sections. A. Staining of mouse olfactory bulb sections with Anti-Prokineticin Receptor 1 (extracellular) antibody (#APR-041), (1:100)(green fluorescence) reveals intensely stained astrocyte-like cells in the glomeruli (G) and lightly stained astrocyte-like cells in the adjacent layer (arrows). B. Merge of images of PKR1 (green), parvalbumin (red, a neuronal marker) and DAPI (blue) nuclear counterstain. |
| Flow Cytometry: |
FACS analysis of Prokineticin Receptor 1 expression in live intact WEHI-231 (mouse B cell lymphoma) cell lines. |
 |
Control cells + Goat-anti-Rabbit-Phycoerythrin (PE) Cells+ Prokineticin Receptor 1 (extracellular) antibody (#APR-041)(1:20) + Goat-anti-Rabbit-PE |
The Prokineticins (PK1 and PK2) are a pair of cysteine-rich secreted peptides with broad physiological functions including gastrointestinal motility, angiogenesis, hematopoiesis and circadian rhythms regulation.
The biological effects of the PK1 and PK2 are mediated by two highly homologous receptors termed Prokineticin Receptor 1 (PKR1) and Prokineticin Receptor 2 (PKR2) that belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. Both PK ligands activate the two PK receptors with similar potency.1
PKR1 receptors couple to Gq/G11 proteins leading to phospholipase C activation, inositol phosphate production and calcium mobilization. In addition, activation of the mitogen-activated protein kinase (MAPK) pathways has also been described. 1
PKR1 is broadly distributed throughout peripheral tissues including the intestinal tract, testis, uterus, lung and peripheral blood leukocytes. In addition, the receptor is expressed in the brain particularly in olfactory regions as well as in dorsal root ganglion (DRG) neurons.
The physiological function of PKR1 is still being elucidated, however, evidence suggest that the receptor has an important role in pain perception through its ability to stimulate the TRPV1 ion channel in DRG neurons.2
In addition, a role for PKR1 in the control of the inflammatory response through regulation of macrophage chemotaxis and cytokine production has also been demonstrated.3
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the extracellular N-terminus domain of the rat PKR1 receptor. The epitope is specific for PKR1 and will not cross-react with the closely related PKR2 receptor. Anti-Prokineticin Receptor 1 (extracellular) antibody (#APR-041) can be used in Western blot, immunohistochemical and flow cytometry applications, and recognizes PKR1 from rat and mouse samples.