Anti-proBDNF
- 貨號 Cat. No.
- ANT-006
- 規格 Size
- 50 μl 0.2ml
Description
Anti-proBDNF
| Product#: | ANT-006 |
| Sizes: |
| 50 ml |
| 0.2 ml |
| Host: |
| Rabbit |
| Epitope: |
| Peptide(C)DEDQKVRPNEENNKDAD, corresponding to amino acid residues 72-88 of human BDNF (precursor) (Accession P23560 ). |
 |
| Putative epitope location: |
| Pro-domain of the BDNF protein. |
| Homology: |
Mouse, rat 16 out of 17 residues identical. |
| Reactivity Confirmed: |
Human, rat, mouse. |
Applications:
| Western Blotting: |
 | Western blotting of rat glioma C6 (1, 3) or human neuroblastoma SH-SY5Y (2, 4) cell lysate: 1, 2. Anti-proBDNF antibody (#ANT-006) (1:200). 3, 4. Anti-proBDNF antibody, preincubated with the control peptide antigen. |
 | Western blotting of mouse recombinant proBDNF: 1 Anti-proBDNF antibody (#ANT-006) (1:400). 2. Anti-proBDNF antibody, preincubated with the control peptide antigen. |
| Immunohistochemistry: |
 |
Staining of pro-brain derived neurotrophic factor (proBDNF) with Anti-proBDNF antibody (#ANT-006) in rat cerebellum. Pro-BDNF (red) appears in Purkinje cell bodies (vertical arrows) and in astrocytic processes (horizontal arrows in A) but not in Purkinje neuronal dendrites stained for calbindin D28k (green, in B) in the same brain section. C, confocal merge of proBDNF and CBD28K demonstrates the restriction of proBDNF to Purkinje cell body. |
| References: |
| 1. Lee R. et al. (2001) Science 294,1945. |
| 2. Egan, M.F. et al. (2003) Cell 112, 257. |
| 3. Pang, P.T. et al. (2004) Science 306, 487. |
| 4. Michalski, B. et al. (2003) Mol. Brain Res. 111, 148. |
Brain derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that includes nerve growth factor (NGF) neurotrophin-3 (NT-3) and neurotrophin-4/5 (NT-4/5).
All neurotrophins are synthesized as preproneurotrophin precursors that are subsequently processed within the intracellular transport pathway to yield proneurotrophins that are further processed to generate the mature form. The mature form of BDNF is a non-covalent stable homodimer that can be secreted in both constitutive and regulated pathways.
Until recently, the functional role of the neurotrophin prodomains were thought to include assistance in the correct folding of the mature protein and the sorting of the neurotrophins into the constitutive or regulated secretory pathway. However, a growing body of evidence suggests that the uncleaved proneurotrophin precursors can be secreted from cells and that they may mediate different biological functions.
The functional importance of the prodomain of BDNF was recently demonstrated in a study showing that a polymorphism that replaces valine for methionine at position 66 of the pro domain, is associated with memory defects and abnormal hippocampal function in humans. Another recent study showed that the regulated extracellular cleavage of proBDNF to mature BDNF by plasmin is necessary for establishing late-phase long-term potentiation (L-LTP) a process that involves long-lasting changes in the structure and function of hippocampal synapses. Finally, proBDNF was shown to be decreased in the brains of patients suffering from Alzheimerî–¸ disease.
Mature BDNF binds to the specific tyrosine kinase receptor TrkB and to p75NTR, a member of the TNF receptor superfamily. ProBDNF can similarly bind both receptors although it appears to have a greater affinity for the p75NTR receptor.
We have now developed a new antibody directed against a highly specific epitope located in the pro domain region of human BDNF. The antibody does not cross react with mature BDNF, pro and mature NGF or mature NT-3.